Human LIF enzyme-linked immunoassay kit

見積もりを依頼する

お問い合わせ

E-mail: [email protected]

TEL: 0571-88062880

代理店・販売店を探す

Catalog# EH0096

Human LIF enzyme-linked immunoassay kit

Application

ELISA

Reactivity

Human

Standard curve range

0.69~500 pg/ml

製品概要

製品名

Human LIF enzyme-linked immunoassay kit

製品タイプ

Elisa Kit

検出感度

0.10 pg/ml (50 μl)；0.36 pg/ml (10 μl)

pg/ml	O.D.		Average	Corrected
0.00	0.0142	0.0145	0.0143
0.69	0.0244	0.0253	0.0248	0.0105
2.06	0.0432	0.0436	0.0434	0.0291
6.17	0.0940	0.0911	0.0926	0.0782
18.52	0.2485	0.2410	0.2448	0.2304
55.56	0.7105	0.6925	0.7015	0.6872
166.67	1.7840	1.6840	1.7340	1.7197
500.00	3.3730	3.4030	3.3880	3.3737

精度

	Intra-Assay Precision			Inter-Assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average (pg/ml)	163.4	48.5	10.5	177.7	61.2	12.2
Standard Deviation	9.6	2.3	0.6	5.4	2.3	0.6
Coefficient of Variation (%)	5.9	4.8	6.2	3.0	3.8	4.9

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

スパイク回収

The spike recovery was evaluated by spiking 3 levels of human LIF into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 93% to 116% with an overall mean recovery of 102%.

サンプル値

Sample Matrix	Sample Evaluated	Range (pg/ml)	Detectable (%)	Mean of Detectable (pg/ml)
Serum	30	1.85-16.87	100	4.94

Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of VE Cadherin in this assay. No medical histories were available for the donors.

実験タイプ

Sandwich Elisa

種属反応性

Human

検出方法

Colorimetric

所要時間

120min

標準曲線梯度

7点/3倍

インキュベーション回数

Detectable Sample

可溶性物质的液相样品。例如：血清，血浆，细胞培养上清，组织研磨液等。

サンプル添加量

50 μl/10 μl

製品の特徴

保存条件

2-8 ℃で保存

ターゲット

UNIPROT #

SwissProt: P15018 Human

別名

CDF

Cholinergic Differentiation Factor

D factor

DIA

Differentiation inducing factor

differentiation inhibitory activity

Differentiation stimulating factor

Differentiation-stimulating factor

Emfilermin

Hepatocyte stimulating factor III

展開

機能

LIF (leukemia inhibitory factor) is a widely expressed, highly and variably glycosylated, 32‑62 kDa, monomeric, pleiotropic cytokine in the IL‑6 family of helical cytokines. The first exon encoding the signal sequence is alternately spliced, resulting in LIF-D, LIF-M, and LIF‑T mRNAs that produce secreted, extracellular matrix‑associated, and intracellular forms, respectively. LIF-D and LIF-M mRNAs produce identical 180 amino acid (aa) mature sequences. Mature human LIF (180 aa) shares 78%, 82%, 91%, 88 and 87% aa sequence identity with mouse, rat, canine, bovine, and porcine LIF, respectively. The LIF receptor is a heterodimer of a type I transmembrane ligand‑binding subunit, LIFR (gp190), and the type I transmembrane signal transducing subunit, gp130, signaling especially through STAT3 and JAK kinases. Gp130 and members of the LIFR family also mediate the biological effects of Oncostatin M, Cardiotrophin‑1, Galectin‑10, CNTF, IL‑6, IL‑11, and IL‑27. A soluble LIFR has been reported in the mouse. Depending on the cells and their context, LIF either opposes or favors differentiation. LIF produced by the uterine endometrium supports successful implantation of the embryo, promotes proliferation and maintenance of pluripotency in embryonic stem cells, and favors proliferation of progenitor cell types such as hematopoietic stem cells. However, excess LIF blocks differentiation of embryoid bodies, indicating the importance of LIF regulation. LIF is produced by CD4+ T cells in response to activation, and is required by the thymic epithelium to support T cell maturation. LIF expression is up‑regulated by neuronal injury, and promotes motor neuron survival and oligodendrocyte myelination. LIF is produced by the adrenal cortex and likely enhances its production of cortisol and aldosterone. LIF can function as an autocrine growth factor in some pancreatic cancers, but induces differentiation in the myeloid leukemic cell line M1. Tumor LIF can also induce formation of immunosuppressive tumor‑associated macrophages. LIF promotes endometrial remodeling and differentiation of adipocytes and cardiac smooth muscle cells. It promotes regulatory T cell and inhibits Th17 cell differentiation, thus promoting tolerance, down‑regulating inflammation, and contributing to immune tolerance during pregnancy and in the nervous system.

Human LIF enzyme-linked immunoassay kit

お問い合わせ

製品概要

製品名

製品タイプ

検出感度

精度

スパイク回収

サンプル値

実験タイプ

種属反応性

検出方法

所要時間

標準曲線梯度

インキュベーション回数

Detectable Sample

サンプル添加量

製品の特徴

保存条件

ターゲット

UNIPROT #

別名

機能

同一ターゲット・同一パスウェイ製品

Human CD47 enzyme-linked immunoassay kit

Human beta Amyloid 1-42 enzyme-linked immunoassay kit

Human/Mouse MASP2 enzyme-linked immunoassay kit

Human SAA1 enzyme-linked immunoassay kit

Human CD254/RANKL/TNFSF11 enzyme-linked immunoassay kit

Human IL-3 enzyme-linked immunoassay kit

Human Complement factor B enzyme-linked immunoassay kit(one step)

Human IL-17E/IL-25 enzyme-linked immunoassay kit

Human Myeloperoxidase(MPO) enzyme-linked immunoassay kit(one step)

Human Leptin enzyme-linked immunoassay kit

Human PAI-1/Serpin E1 enzyme-linked immunoassay kit(one step)

Human ULBP1/NKG2DL1 enzyme-linked immunoassay kit

Human CD273/B7-DC/PD-L2 enzyme-linked immunoassay kit（one step）

Human VE Cadherin enzyme-linked immunoassay kit

Human CD62E enzyme-linked immunoassay kit

Human/Monkey alpha Fetoprotein enzyme-linked immunoassay kit

Human CX3CL1/Fractalking enzyme-linked immunoassay kit

Human/Monkey PCSK9 enzyme-linked immunoassay kit (one step)

Human Growth Hormone enzyme-linked immunoassay kit

Human Nectin-2 enzyme-linked immunoassay kit

Human/Monkey/Canine Osteopontin（OPN） enzyme-linked immunoassay kit

Human Total HSP60 enzyme-linked immunoassay kit

Human Angiotensinogen enzyme-linked immunoassay kit

Human IL-33 enzyme-linked immunoassay kit

Human RAGE enzyme-linked immunoassay kit（one step）

Human Prostate specific Antigen/KLK3 enzyme-linked immunoassay kit

Human CD48 enzyme-linked immunoassay kit

Human/Monkey Albumin enzyme-linked immunoassay kit(one step)

Human IL-13 enzyme-linked immunoassay kit

Human B7-H2/CD275 enzyme-linked immunoassay kit

Human Alpha-Synuclein enzyme-linked immunoassay kit

Human MMP-3 enzyme-linked immunoassay kit

Human MERTK enzyme-linked immunoassay kit（one step）

Human Adiponectinenzyme-linked immunoassay kit（one step）

Human CCL25 enzyme-linked immunoassay kit

Human TREM-1 enzyme-linked immunoassay kit

Human Lipoprotein A/LPA enzyme-linked immunoassay kit(one step)

Human TNF RI/TNFRSF1A enzyme-linked immunoassay kit

Human Lipoprotein A/LPA enzyme-linked immunoassay kit

Human MICB enzyme-linked immunoassay kit

Human SCF/KITLG enzyme-linked immunoassay kit

Human CCL17/TARC enzyme-linked immunoassay kit

Human/Monkey/Canine IL-18 enzyme-linked immunoassay kit

Human tPA enzyme-linked immunoassay kit(one step)

Human Antithrombin III/AT III enzyme-linked immunoassay kit(one step)

Human TSLP enzyme-linked immunoassay kit

Human/Monkey Clusterin enzyme-linked immunoassay kit(one step)

Human FGF-19 enzyme-linked immunoassay kit

Human/Monkey Complement C3 enzyme-linked immunoassay kit(one step)

Human CD33 enzyme-linked immunoassay kit

Human Oxytocin-neurophysin 1/OXT enzyme-linked immunoassay kit

Human CXCL5/ENA-78 enzyme-linked immunoassay kit

Human/Monkey/Canine CXCL10/IP-10 enzyme-linked immunoassay kit

Human CTLA-4 enzyme-linked immunoassay kit

Human CHIT1 enzyme-linked immunoassay kit

Human Angiogenin enzyme-linked immunoassay kit（one step）