Human Mesothelin enzyme-linked immunoassay kit(one step)
Catalog# EHY0152
Human Mesothelin enzyme-linked immunoassay kit(one step)
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ELISA
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Human
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1.37~1000 ng/ml
製品概要
製品名
Human Mesothelin enzyme-linked immunoassay kit(one step)
製品タイプ
Elisa Kit
検出感度
0.16 ng/ml (50 μl)；0.23 ng/ml (10 μl)；
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0128 | 0.0150 | 0.0139 | |
| 1.37 | 0.0231 | 0.0235 | 0.0233 | 0.0094 |
| 4.12 | 0.0455 | 0.0442 | 0.0449 | 0.0310 |
| 12.35 | 0.1127 | 0.1187 | 0.1157 | 0.1018 |
| 37.04 | 0.3142 | 0.3075 | 0.3109 | 0.2970 |
| 111.11 | 0.8870 | 0.9468 | 0.9169 | 0.9030 |
| 333.33 | 2.5550 | 2.4420 | 2.4985 | 2.4846 |
| 1000.00 | 4.3296 | 4.4273 | 4.3785 | 4.3646 |
精度
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (ng/ml) | 22.2 | 107.5 | 332.8 | 19.3 | 97.4 | 295.0 |
| Standard Deviation | 0.8 | 4.5 | 13.4 | 0.8 | 3.5 | 9.2 |
| Coefficient of Variation (%) | 3.5 | 4.2 | 4.0 | 3.9 | 3.5 | 3.1 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
スパイク回収
The spike recovery was evaluated by spiking 3 levels of human Mesothelin into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 85% to 103% with an overall mean recovery of 94%.
サンプル値
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 51.87-224.14 | 100 | 119.25 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of Mesothelin in this assay. No medical histories were available for the donors.
実験タイプ
種属反応性
検出方法
所要時間
標準曲線梯度
インキュベーション回数
2
Detectable Sample
サンプル添加量
製品の特徴
保存条件
2-8 ℃で保存
ターゲット
UNIPROT #
別名
CAK 1
CAK1
CAK1 antigen
cleaved form
Megakaryocyte potentiating factor
Mesothelin
Mesothelin isoform 1 precursor
MPF
Msln
MSLN_HUMAN
展開