Human IFN-β enzyme-linked immunoassay kit
Catalog# EH0017
Human IFN-β enzyme-linked immunoassay kit
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ELISA
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Human
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15.63~1000 pg/ml
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unconjugated
製品概要
製品名
Human IFN-β enzyme-linked immunoassay kit
製品タイプ
Elisa Kit
検出感度
0.35 pg/ml (50 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0349 | 0.0318 | 0.0334 | |
| 15.63 | 0.0798 | 0.0747 | 0.0773 | 0.0439 |
| 31.25 | 0.1220 | 0.1323 | 0.1272 | 0.0938 |
| 62.50 | 0.2001 | 0.1991 | 0.1996 | 0.1663 |
| 125.00 | 0.3930 | 0.4183 | 0.4057 | 0.3723 |
| 250.00 | 0.8126 | 0.7841 | 0.7984 | 0.7650 |
| 500.00 | 1.7670 | 1.6420 | 1.7045 | 1.6712 |
| 1000.00 | 3.5520 | 3.5080 | 3.5300 | 3.4967 |
精度
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 20.0 | 99.1 | 304.4 | 21.3 | 101.4 | 317.2 |
| Standard Deviation | 1.2 | 5.8 | 14.3 | 1.5 | 4.2 | 15.7 |
| Coefficient of Variation (%) | 6.2 | 5.9 | 4.7 | 7.1 | 4.1 | 5.0 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
スパイク回収
The spike recovery was evaluated by spiking 3 levels of human IFN beta into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 102% to 129% with an overall mean recovery of 121%.
サンプル値
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-2.08 | 53 | 1.03 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of IFN-β in this assay. No medical histories were available for the donors.
実験タイプ
種属反応性
検出方法
所要時間
標準曲線梯度
インキュベーション回数
2
Detectable Sample
サンプル添加量
製品の特徴
保存条件
2-8 ℃で保存
文献におけるアプリケーション
| ELISA | 下記の<%= count %>篇の文献を見る |
文献におけるアプリケーション
| Human | 下記の<%= count %>篇の文献を見る |
ターゲット
UNIPROT #
別名
機能
文献・引用論文
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Development and Comprehensive Evaluation of Scarless Circularization Systems for Circular RNA Therapeutics
ジャーナル: Molecular Therapy-Nucleic Acids
DOI: 10.1016/j.omtn.2025.102587
IF: 6.1
アプリケーション: ELISA
交差反応性: Human
掲載日: 2025 Jun
-
The cGAS-STING pathway activates transcription factor TFEB to stimulate lysosome biogenesis and pathogen clearance
ジャーナル: Immunity
DOI:
IF: 25.5
アプリケーション: ELISA
交差反応性: Human
掲載日: 2024 Dec