Human M-CSF enzyme-linked immunoassay kit

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Catalog# EH0126

Human M-CSF enzyme-linked immunoassay kit

Application

ELISA

Reactivity

Human

Standard curve range

78.13~5000 pg/ml

製品概要

製品名

Human M-CSF enzyme-linked immunoassay kit

製品タイプ

Elisa Kit

検出感度

1.41 pg/ml (50 μl)；15.89 pg/ml (10 μl)

pg/ml	O.D.		Average	Corrected
0.00	0.0157	0.0157	0.0157
78.13	0.0691	0.0610	0.0651	0.0494
156.25	0.1317	0.1102	0.1210	0.1053
312.50	0.2432	0.2484	0.2458	0.2301
625.00	0.4781	0.4755	0.4768	0.4611
1250.00	0.9490	0.9950	0.9720	0.9563
2500.00	2.0360	1.7810	1.9085	1.8928
5000.00	3.3130	3.2000	3.2565	3.2408

精度

	Intra-Assay Precision			Inter-Assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average (pg/ml)	165.6	689.8	2146.1	110.7	548.3	1660.5
Standard Deviation	9.8	42.2	119.7	5.2	37.6	83.2
Coefficient of Variation (%)	5.9	6.1	5.6	4.7	6.9	5.0

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

スパイク回収

The spike recovery was evaluated by spiking 3 levels of human M-CSF into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 72% to 121% with an overall mean recovery of 105%.

サンプル値

Sample Matrix	Sample Evaluated	Range (pg/ml)	Detectable (%)	Mean of Detectable (pg/ml)
Serum	30	684.79-1505.39	100	1024.43

Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of CD137/4-1BB/TNFRSF9 in this assay. No medical histories were available for the donors.

実験タイプ

Sandwich Elisa

種属反応性

Human

検出方法

Colorimetric

所要時間

120min

標準曲線梯度

7点

インキュベーション回数

Detectable Sample

可溶性物质的液相样品。例如：血清，血浆，细胞培养上清，组织研磨液等。

サンプル添加量

50 μl/10 μl

製品の特徴

保存条件

2-8 ℃で保存

ターゲット

UNIPROT #

SwissProt: P09603 Human

別名

Colony stimulating factor 1 (macrophage)

Colony stimulating factor 1

Colony stimulating factor macrophage specific

CSF 1

CSF-1

CSF1

CSF1_HUMAN

Csfm

Lanimostim

M CSF

展開

機能

M-CSF, also known as CSF-1, is a four-alpha -helical-bundle cytokine that is the primary regulator of macrophage survival, proliferation and differentiation. M-CSF protein is also essential for the survival and proliferation of osteoclast progenitors. M-CSF also primes and enhances macrophage killing of tumor cells and microorganisms, regulates the release of cytokines and other inflammatory modulators from macrophages, and stimulates pinocytosis. M-CSF increases during pregnancy to support implantation and growth of the decidua and placenta. Sources of M-CSF include fibroblasts, activated macrophages, endometrial secretory epithelium, bone marrow stromal cells and activated endothelial cells . The M-CSF receptor (c-fms) transduces its pleotropic effects and mediates its endocytosis. M-CSF mRNAs of various sizes occur. Full length mouse M-CSF transcripts encode a 520 amino acid (aa) type I transmembrane (TM) protein with a 462 aa extracellular region, a 21 aa TM domain, and a 37 aa cytoplasmic tail that forms a 140 kDa covalent dimer. Differential processing produces two proteolytically cleaved, secreted dimers. One is an N- and O- glycosylated 86 kDa dimer, while the other is modified by both glycosylation and chondroitin-sulfate proteoglycan (PG) to generate a 200 kDa subunit. Although PG-modified M-CSF protein can circulate, it may be immobilized by attachment to type V collagen . Shorter transcripts encode M‑CSF that lacks cleavage and PG sites and produces an N-glycosylated 68 kDa TM dimer and a slowly produced 44 kDa secreted dimer. Although forms may vary in activity and half-life, all contain the N-terminal 150 aa portion that is necessary and sufficient for interaction with the M-CSF receptor. The first 229 aa of mature mouse M-CSF shares 87%, 83%, 82% and 81% aa identity with corresponding regions of rat, dog, cow and human M-CSF, respectively. Human M‑CSF is active in the mouse, but mouse M-CSF is reported to be species-specific.

Human M-CSF enzyme-linked immunoassay kit

お問い合わせ

製品概要

製品名

製品タイプ

検出感度

精度

スパイク回収

サンプル値

実験タイプ

種属反応性

検出方法

所要時間

標準曲線梯度

インキュベーション回数

Detectable Sample

サンプル添加量

製品の特徴

保存条件

ターゲット

UNIPROT #

別名

機能

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