Human CXCL5/ENA-78 enzyme-linked immunoassay kit
Catalog# EH0217
Human CXCL5/ENA-78 enzyme-linked immunoassay kit
-
ELISA
-
Human
-
20.48~5000 pg/ml
製品概要
製品名
Human CXCL5/ENA-78 enzyme-linked immunoassay kit
製品タイプ
Elisa Kit
検出感度
0.29 pg/ml (50 μl)；2.99 pg/ml (10 μl)；
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0134 | 0.0136 | 0.0135 | |
| 20.48 | 0.0370 | 0.0376 | 0.0373 | 0.0238 |
| 51.20 | 0.0745 | 0.0774 | 0.0760 | 0.0625 |
| 128.00 | 0.1724 | 0.1706 | 0.1715 | 0.1580 |
| 320.00 | 0.4228 | 0.4138 | 0.4183 | 0.4048 |
| 800.00 | 1.0020 | 1.0140 | 1.0080 | 0.9945 |
| 2000.00 | 2.2430 | 2.1530 | 2.1980 | 2.1845 |
| 5000.00 | 3.9350 | 3.8410 | 3.8880 | 3.8745 |
精度
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 135.3 | 659.5 | 1976.8 | 111.1 | 538.6 | 1723.8 |
| Standard Deviation | 8.7 | 37.6 | 102.5 | 4.3 | 17.4 | 78.9 |
| Coefficient of Variation (%) | 6.4 | 5.7 | 5.2 | 3.9 | 3.2 | 4.6 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
スパイク回収
The spike recovery was evaluated by spiking 3 levels of human CXCL5/ENA-78 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 106% with an overall mean recovery of 89%.
サンプル値
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 1.93-7.91 | 100 | 3.83 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of CXCL5/ENA-78 in this assay. No medical histories were available for the donors.
実験タイプ
種属反応性
検出方法
所要時間
標準曲線梯度
インキュベーション回数
2
Detectable Sample
サンプル添加量
製品の特徴
保存条件
2-8 ℃で保存
ターゲット
UNIPROT #
別名
AMCFII
C-X-C motif chemokine 5
C-X-C motif chemokine ligand 5
Chemokine (C X C motif) ligand 5
chemokine (C-X-C motif) ligand 5
Cxcl5
CXCL5_HUMAN
ENA 78
ENA-78 (8-78)
ENA-78(1-78)
展開