Human Neurogranin enzyme-linked immunoassay kit

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Catalog# EH0210

Human Neurogranin enzyme-linked immunoassay kit

Application

ELISA

Reactivity

Human

Standard curve range

8.19~2000 pg/ml

製品概要

製品名

Human Neurogranin enzyme-linked immunoassay kit

製品タイプ

Elisa Kit

検出感度

0.92 pg/ml (50 μl)；11.03 pg/ml (10 μl)；

pg/ml	O.D.		Average	Corrected
0.00	0.0112	0.0104	0.0108
8.19	0.0162	0.0174	0.0168	0.0060
20.48	0.0261	0.0273	0.0267	0.0159
51.20	0.0538	0.0552	0.0545	0.0437
128.00	0.1319	0.1439	0.1379	0.1271
320.00	0.3888	0.4207	0.4048	0.3940
800.00	1.3030	1.2710	1.2870	1.2762
2000.00	3.5340	3.5620	3.5480	3.5372

精度

	Intra-Assay Precision			Inter-Assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average (pg/ml)	31.3	163.1	551.2	35.6	175.0	570.3
Standard Deviation	1.8	8.7	23.9	1.5	5.2	10.0
Coefficient of Variation (%)	5.8	5.3	4.3	4.3	3.0	1.7

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

スパイク回収

The spike recovery was evaluated by spiking 3 levels of human Neurogranin into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 119% with an overall mean recovery of 106%.

サンプル値

Sample Matrix	Sample Evaluated	Range (pg/ml)	Detectable (%)	Mean of Detectable (pg/ml)
Serum	30	n.d.-9.44	67	2.21

Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of Neurogranin in this assay. No medical histories were available for the donors.

実験タイプ

Sandwich Elisa

種属反応性

Human

検出方法

Colorimetric

所要時間

120min

標準曲線梯度

7点

インキュベーション回数

Detectable Sample

可溶性物质的液相样品。例如：血清，血浆，细胞培养上清，组织研磨液等。

サンプル添加量

50 μl/10 μl

製品の特徴

保存条件

2-8 ℃で保存

ターゲット

UNIPROT #

SwissProt: Q92686 Human

別名

Protein kinase C substrate RC3

Calmodulin binding protein

Hng

NEUG(55-78)

NEUG_HUMAN

Neurogranin (protein kinase C substrate)

NRGN

Protein kinase C substrate

RC3

機能

NRGN (Neurogranin/Ng; also RC3, p17 and BICKS) is a member of the neurogranin family of proteins. Although its predicted MW is 7.5 kDa, it runs anomalously at 15-19 kDa in SDS-PAGE. This is apparently due to the adoption of a rigid alpha -helical structure in a negatively charged medium. NRGN has limited expression, being found principally in excitatory neurons of the telencephalon, Golgi and Purkinje cells of the cerebellum, and platelets plus B and T cells in non-nervous tissue. Intracellularly, NRGN is found associated with membranes of the ER, Golgi and mitochondria. This association is often in the form of aggregates (or granules), thus giving rise to its name ("neuro-granules"). NRGN is also found in the nucleus and associated with the postsynaptic spines of dendrites. The principal function of NRGN appears to be the binding, sequestration and concentration of CaM (calmodulin; a Ca-binding protein) in dendritic spines. Following NMDAR activation, Ca diffuses into the synaptic area, resulting in 1) the simply dissociation of CaM from NRGN, or 2) the phosphorylation of NRGN followed by its dissociation fro CaM. In either case, the freed CaM is now available to activate multiple downstream signaling pathways, some involved in LTP (or memory). Human NRGN is 78 amino acids (aa) in length. It contains one IQ domain (aa 26-47) that binds CaM, and a collagen-like region at the C-terminus (aa 48-78). Regulatory phosphorylation occurs on Ser36, and the N-terminal Met is acetylated. Full-length human NRGN shares 96% aa sequence identity with both mouse and rat NRGN.

Human Neurogranin enzyme-linked immunoassay kit

お問い合わせ

製品概要

製品名

製品タイプ

検出感度

精度

スパイク回収

サンプル値

実験タイプ

種属反応性

検出方法

所要時間

標準曲線梯度

インキュベーション回数

Detectable Sample

サンプル添加量

製品の特徴

保存条件

ターゲット

UNIPROT #

別名

機能

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