Human TPO/Thrombopoietin enzyme-linked immunoassay kit

見積もりを依頼する

お問い合わせ

E-mail: [email protected]

TEL: 0571-88062880

代理店・販売店を探す

Catalog# EH0197

Human TPO/Thrombopoietin enzyme-linked immunoassay kit

Application

ELISA

Reactivity

Human

Standard curve range

8.19~2000 pg/ml

製品概要

製品名

Human TPO/Thrombopoietin enzyme-linked immunoassay kit

製品タイプ

Elisa Kit

検出感度

0.12 pg/ml (50 μl)；7.98 pg/ml (10 μl)；

pg/ml	O.D.		Average	Corrected
0.00	0.0260	0.0252	0.0256
8.19	0.0460	0.0467	0.0464	0.0208
20.48	0.0769	0.0723	0.0746	0.0490
51.20	0.1445	0.1427	0.1436	0.1180
128.00	0.3214	0.3069	0.3142	0.2886
320.00	0.6924	0.6953	0.6939	0.6683
800.00	1.6190	1.5460	1.5825	1.5569
2000.00	3.2320	3.2120	3.2220	3.1964

精度

	Intra-Assay Precision			Inter-Assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average (pg/ml)	40.7	197.2	578.0	37.2	208.0	617.8
Standard Deviation	2.9	9.9	34.8	2.4	11.7	41.3
Coefficient of Variation (%)	7.1	5.0	6.0	6.5	5.6	6.7

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

スパイク回収

The spike recovery was evaluated by spiking 3 levels of human TPO/Thrombopoietin into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 88% to 128% with an overall mean recovery of 114%.

サンプル値

Sample Matrix	Sample Evaluated	Range (pg/ml)	Detectable (%)	Mean of Detectable (pg/ml)
Serum	30	5.60-1005.88	100	253.32

Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of TPO/Thrombopoietin in this assay. No medical histories were available for the donors.

実験タイプ

Sandwich Elisa

種属反応性

Human

検出方法

Colorimetric

所要時間

120min

標準曲線梯度

7点

インキュベーション回数

Detectable Sample

可溶性物质的液相样品。例如：血清，血浆，细胞培养上清，组织研磨液等。

サンプル添加量

50 μl/10 μl

製品の特徴

保存条件

2-8 ℃で保存

ターゲット

UNIPROT #

SwissProt: P40225 Human

別名

C mpl ligand

C-mpl ligand

Megakaryocyte colony stimulating factor

Megakaryocyte colony-stimulating factor

Megakaryocyte growth and development factor

Megakaryocyte stimulating factor

MGC163194

MGDF

MKCSF

展開

機能

Thrombopoietin (Tpo), is a key regulator of megakaryocytopoiesis and thrombopoiesis. It is principally produced in the liver and is bound and internalized by the receptor Tpo R/c-mpl. Defects in the Tpo-Tpo R signaling pathway are associated with a variety of platelet disorders. The 356 amino acid (aa) mouse Tpo precursor is cleaved to yield the 335 aa mature protein. Mature mouse Tpo shares 71% and 81% aa sequence homology with human and rat Tpo, respectively. It is an 80‑85 kDa protein that consists of an N-terminal domain with homology to Erythropoietin (Epo) and a C‑terminal domain that contains multiple N-linked and O-linked glycosylation sites. Tissue specific alternate splicing of mouse Tpo generates multiple isoforms with internal deletions, insertions, and/or C‑terminal substitutions. Tpo promotes the differentiation, proliferation, and maturation of MK and their progenitors. Several other cytokines can promote these functions as well but only in cooperation with Tpo. Notably, IL-3 independently induces MK development, although its effects are restricted to early in the MK lineage. Tpo additionally promotes platelet production, aggregation, ECM adhesion, and activation. It is cleaved by platelet-derived thrombin following Arg191 within the C‑terminal domain and subsequently at other sites upon extended digestion. Full length Tpo and shorter forms circulate in the plasma. The C‑terminal domain is not required for binding to Tpo R or inducing MK growth and differentiation. Aside from its hematopoietic effects, Tpo is expressed in the brain where it promotes the apoptosis of hypoxia-sensitized neurons and inhibits neuronal differentiation by blocking NGF-induced signaling.

Human TPO/Thrombopoietin enzyme-linked immunoassay kit

お問い合わせ

製品概要

製品名

製品タイプ

検出感度

精度

スパイク回収

サンプル値

実験タイプ

種属反応性

検出方法

所要時間

標準曲線梯度

インキュベーション回数

Detectable Sample

サンプル添加量

製品の特徴

保存条件

ターゲット

UNIPROT #

別名

機能

同一ターゲット・同一パスウェイ製品

Human CD47 enzyme-linked immunoassay kit

Human beta Amyloid 1-42 enzyme-linked immunoassay kit

Human/Mouse MASP2 enzyme-linked immunoassay kit

Human SAA1 enzyme-linked immunoassay kit

Human CD254/RANKL/TNFSF11 enzyme-linked immunoassay kit

Human IL-3 enzyme-linked immunoassay kit

Human Complement factor B enzyme-linked immunoassay kit(one step)

Human IL-17E/IL-25 enzyme-linked immunoassay kit

Human Myeloperoxidase(MPO) enzyme-linked immunoassay kit(one step)

Human Leptin enzyme-linked immunoassay kit

Human PAI-1/Serpin E1 enzyme-linked immunoassay kit(one step)

Human ULBP1/NKG2DL1 enzyme-linked immunoassay kit

Human CD273/B7-DC/PD-L2 enzyme-linked immunoassay kit（one step）

Human VE Cadherin enzyme-linked immunoassay kit

Human CD62E enzyme-linked immunoassay kit

Human/Monkey alpha Fetoprotein enzyme-linked immunoassay kit

Human CX3CL1/Fractalking enzyme-linked immunoassay kit

Human/Monkey PCSK9 enzyme-linked immunoassay kit (one step)

Human Growth Hormone enzyme-linked immunoassay kit

Human Nectin-2 enzyme-linked immunoassay kit

Human/Monkey/Canine Osteopontin（OPN） enzyme-linked immunoassay kit

Human Total HSP60 enzyme-linked immunoassay kit

Human Angiotensinogen enzyme-linked immunoassay kit

Human IL-33 enzyme-linked immunoassay kit

Human RAGE enzyme-linked immunoassay kit（one step）

Human Prostate specific Antigen/KLK3 enzyme-linked immunoassay kit

Human CD48 enzyme-linked immunoassay kit

Human/Monkey Albumin enzyme-linked immunoassay kit(one step)

Human IL-13 enzyme-linked immunoassay kit

Human B7-H2/CD275 enzyme-linked immunoassay kit

Human Alpha-Synuclein enzyme-linked immunoassay kit

Human MMP-3 enzyme-linked immunoassay kit

Human MERTK enzyme-linked immunoassay kit（one step）

Human Adiponectinenzyme-linked immunoassay kit（one step）

Human CCL25 enzyme-linked immunoassay kit

Human Lipoprotein A/LPA enzyme-linked immunoassay kit(one step)

Human TNF RI/TNFRSF1A enzyme-linked immunoassay kit

Human TREM-1 enzyme-linked immunoassay kit

Human Lipoprotein A/LPA enzyme-linked immunoassay kit

Human tPA enzyme-linked immunoassay kit(one step)

Human MICB enzyme-linked immunoassay kit

Human Antithrombin III/AT III enzyme-linked immunoassay kit(one step)

Human TSLP enzyme-linked immunoassay kit

Human/Monkey Clusterin enzyme-linked immunoassay kit(one step)

Human SCF/KITLG enzyme-linked immunoassay kit

Human CD33 enzyme-linked immunoassay kit

Human CXCL5/ENA-78 enzyme-linked immunoassay kit

Human/Monkey/Canine CXCL10/IP-10 enzyme-linked immunoassay kit

Human CHIT1 enzyme-linked immunoassay kit

Human CCL17/TARC enzyme-linked immunoassay kit

Human/Monkey/Canine IL-18 enzyme-linked immunoassay kit

Human FGF-19 enzyme-linked immunoassay kit

Human/Monkey Complement C3 enzyme-linked immunoassay kit(one step)

Human IL-6 Super Sensitivity enzyme-linked immunoassay kit

Human Oxytocin-neurophysin 1/OXT enzyme-linked immunoassay kit

Human Proprotein Convertase 9/PCSK9 enzyme-linked immunoassay kit（one step）