Human EGFR enzyme-linked immunoassay kit

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Catalog# EH0254

Human EGFR enzyme-linked immunoassay kit

Application

ELISA

Reactivity

Human

Standard curve range

312.50~20000 pg/ml

製品概要

製品名

Human EGFR enzyme-linked immunoassay kit

製品タイプ

Elisa Kit

検出感度

39.85 pg/ml (50 μl)；59.07 pg/ml (10 μl)；

pg/ml	O.D.		Average	Corrected
0.00	0.0525	0.0456	0.0491
312.50	0.1030	0.1055	0.1043	0.0552
625.00	0.1569	0.1681	0.1625	0.1135
1250.00	0.2733	0.2743	0.2738	0.2248
2500.00	0.4858	0.4679	0.4769	0.4278
5000.00	0.8540	0.9100	0.8820	0.8330
10000.00	1.6050	1.7460	1.6755	1.6265
20000.00	2.8520	3.0830	2.9675	2.9185

精度

	Intra-Assay Precision			Inter-Assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average (pg/ml)	5889.10	1900.99	368.32	5734.9	1945.4	394.4
Standard Deviation	371.92	147.54	24.85	197.9	133.0	31.0
Coefficient of Variation (%)	6.3	7.8	6.7	3.5	6.8	7.9

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

スパイク回収

The spike recovery was evaluated by spiking 3 levels of human EGFR into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 87% to 120% with an overall mean recovery of 108%.

サンプル値

Sample Matrix	Sample Evaluated	Range (ng/ml)	Detectable (%)	Mean of Detectable (ng/ml)
Serum	30	18.68-40.63	100.0	31.80

Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated in this assay. No medical histories were available for the donors.

実験タイプ

Sandwich Elisa

種属反応性

Human

検出方法

Colorimetric

所要時間

120min

標準曲線梯度

7点

インキュベーション回数

Detectable Sample

可溶性物质的液相样品。例如：血清，血浆，细胞培养上清，组织研磨液等。

サンプル添加量

50 μl/10 μl

製品の特徴

保存条件

2-8 ℃で保存

ターゲット

UNIPROT #

SwissProt: P00533 Human

機能

Epidermal growth factor receptor (EGFR), also known as ErbB1 and HER1, is a type I glycoprotein that belongs the ErbB subfamily of receptor tyrosine kinases (RTKs), which includes ErbB2/HER2, ErbB3/HER3, and ErbB4/HER4 . EGFR plays an important role in epithelial cell development and homeostasis and as a driver of tumorigenesis in cancer. The human EGFR is protein 1210 amino acids (aa) in length with a theoretical molecular weight (MW) of 134 kDa. The protein consists of a short signal peptide, an extracellular domain (ECD) divided into four subdomains (I-IV), a transmembrane region, an intracellular juxtamembrane segment, a tyrosine kinase domain, and C-terminal tail. Within the ECD, human EGFR has 88-90% aa sequence identity with mouse and rat EGFR. EGFR has four known specific ligands: EGF, amphiregulin, epigen, and transforming growth factor alpha (TGF-alpha). EGFR ligands betacellulin, epiregulin, and herapin binding (HB)-EGF have dual specificity with ErbB4. Ligand binding to the extracellular domain of EFGR leads to receptor homodimerization, or heterodimerization with other ErbB family members, and EGFR activation. This results in subsequent phosphorylation and activation of intracellular signaling pathways, such as MAPK and PI3K/Akt. EGFR signaling is essential for many cellular processes including proliferation, differentiation, migration, and apoptosis. In addition to its role in normal development, EGFR mutations or overexpression is observed in many tumors, including breast cancer, non-small cell lung carcinoma (NSCLC), colon cancer, and more. Small molecule tyrosine kinase inhibitors (TKIs), like gefitinib, erlotinib, and afatinib, have shown great efficacy in treating patients with EGFR activating mutations, especially for NSCLC. However, most patients eventually develop acquired resistance to TKIs and thus combination and alternative therapies are in development. A third-generation TKI, osimertinib, is approved for NSCLC patients with resistance to first-line EGFR TKI treatment. Additionally, combination therapies of EGFR TKIs with monoclonal antibody immunotherapies, like anti-PD-L1, are being further investigated in clinical trials.

Human EGFR enzyme-linked immunoassay kit

お問い合わせ

製品概要

製品名

製品タイプ

検出感度

精度

スパイク回収

サンプル値

実験タイプ

種属反応性

検出方法

所要時間

標準曲線梯度

インキュベーション回数

Detectable Sample

サンプル添加量

製品の特徴

保存条件

ターゲット

UNIPROT #

機能

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