Human SNAP25 enzyme-linked immunoassay kit
Catalog# EH0228
Human SNAP25 enzyme-linked immunoassay kit
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ELISA
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Human
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6.86~5000 pg/ml
製品概要
製品名
Human SNAP25 enzyme-linked immunoassay kit
製品タイプ
Elisa Kit
検出感度
0.18 pg/ml (50 μl)；2.61 pg/ml (10 μl)；
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0086 | 0.0097 | 0.0092 | |
| 6.86 | 0.0264 | 0.0274 | 0.0269 | 0.0178 |
| 20.58 | 0.0653 | 0.0679 | 0.0666 | 0.0575 |
| 61.73 | 0.1779 | 0.1843 | 0.1811 | 0.1720 |
| 185.19 | 0.5479 | 0.5375 | 0.5427 | 0.5336 |
| 555.56 | 1.4790 | 1.4530 | 1.4660 | 1.4569 |
| 1666.67 | 3.2017 | 3.1982 | 3.2000 | 3.1908 |
| 5000.00 | 4.3728 | 4.4603 | 4.4166 | 4.4074 |
精度
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 97.4 | 500.6 | 1577.5 | 98.0 | 532.5 | 1626.4 |
| Standard Deviation | 5.8 | 31.3 | 86.8 | 3.8 | 34.7 | 93.8 |
| Coefficient of Variation (%) | 6.0 | 6.2 | 5.5 | 3.9 | 6.5 | 5.8 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
スパイク回収
The spike recovery was evaluated by spiking 3 levels of Human SNAP25 into health serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 97% to 106% with an overall mean recovery of 101%.
サンプル値
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-2.30 | 65 | 1.71 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of SNAP25 in this assay. No medical histories were available for the donors.
実験タイプ
種属反応性
検出方法
所要時間
標準曲線梯度
インキュベーション回数
2
Detectable Sample
サンプル添加量
製品の特徴
保存条件
2-8 ℃で保存
ターゲット
UNIPROT #
別名
bA416N4.2
Bdr
CMS18
dJ1068F16.2
FLJ23079
HGNC:11132
MGC105414
MGC139754
Resistance to inhibitors of cholinesterase 4 homolog
RIC 4
展開