Human IL-8/CXCL8 enzyme-linked immunoassay kit

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Catalog# EH0014

Human IL-8/CXCL8 enzyme-linked immunoassay kit

Application

ELISA

Reactivity

Human

Standard curve range

4.12~3000 pg/ml

Conjugation

unconjugated

This product has been cited in peer reviewed publications, see list HERE

製品概要

製品名

Human IL-8/CXCL8 enzyme-linked immunoassay kit

製品タイプ

Elisa Kit

検出感度

1.91 pg/ml (50 μl) ； 2.51 pg/ml (10 μl)

pg/ml	O.D.		Average	Corrected
0.00	0.0572	0.0561	0.0567
4.12	0.1574	0.1630	0.1602	0.1036
12.35	0.2578	0.2664	0.2621	0.2055
37.04	0.5108	0.5064	0.5086	0.4520
111.11	0.8509	0.8440	0.8475	0.7908
333.33	1.5335	1.5718	1.5527	1.4960
1000.00	2.7483	2.7297	2.7390	2.6824
3000.00	3.9326	3.9554	3.9440	3.8874

精度

	Intra-Assay Precision			Inter-Assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average (pg/ml)	59.4	303.5	910.1	57.1	304.5	902.4
Standard Deviation	0.5	5.8	23.9	1.2	9.8	33.2
Coefficient of Variation (%)	2.9	5.6	4.7	3.5	4.3	5.1

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

スパイク回収

The spike recovery was evaluated by spiking 3 levels of human IL-8 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 88% to 112% with an overall mean recovery of 97%.

サンプル値

Sample Matrix	Sample Evaluated	Range (pg/ml)	Detectable (%)	Mean of Detectable (pg/ml)
Serum	30	113.36-	1751.50	100	741.07

Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of IL-8 in this assay. No medical histories were available for the donors.

実験タイプ

Sandwich Elisa

種属反応性

Human

検出方法

Colorimetric

所要時間

120min

標準曲線梯度

7点/3倍

インキュベーション回数

Detectable Sample

可溶性物质的液相样品。例如：血清，血浆，细胞培养上清，组织研磨液等。

サンプル添加量

50 μl/10 μl

製品の特徴

保存条件

2-8 ℃で保存

文献におけるアプリケーション

ELISA

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文献におけるアプリケーション

Human

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ターゲット

UNIPROT #

SwissProt: P10145 Human

別名

(Ala-IL-8)77

(Ser-IL-8)72

9E3

Beta thromboglobulin like protein

C-X-C motif chemokine 8

CEF-4

chemokine, CXC motif, ligand 8

CXCL8

Emoctakin

GCP-1

展開

機能

Interleukin-8 (IL-8), also known as IL-8, GCP-1, and NAP-1, is a heparin-binding 8-9 kDa member of the alpha, or CXC family of chemokines. There are at least 15 human CXC family members that all adopt a three beta -sheet/one alpha -helix structure. Most CXC chemokines show an N-terminal Glu-Leu-Arg (ELR) tripeptide motif. IL-8 circulates as a monomer, homodimer, and heterodimer with CXCL4/PF4. The monomer is considered the most bio-active, while the heterodimer can potentiate PF4 activity. IL-8 oligomerization is modulated by its interactions with matrix and cell surface glycosaminoglycans (GAGs). Mature human IL-8 shares 65-69% amino acid (aa) identity with canine, feline, and porcine IL-8. There is no IL-8 gene counterpart in rodent. Multiple isoforms of IL-8 are generated through both alternative splicing and differential proteolytic cleavage. In humans, alternative splicing generates an iso-form with an eleven aa substitution at the C-terminus. Proteolytic processing results in N-terminal truncation of IL-8 and is likely a cell-specific event. For example, fibroblasts and endothelial cells generate the 1-77 form by cleaving IL-8 following Glu21, while monocytes and lymphocytes generate the 6-77 form by cleaving following Leu25. These truncated forms generally show increased bioactivity, particularly through the CXCR1 receptor. IL-8 can also undergo citrullination on Arg27 of the precursor, a modification that increases its half-life and ability to induce leukocytosis. A wide variety of cells secrete IL-8 including monocytes and neutrophils, fibroblasts and keratinocytes, mast cells, visceral smooth muscle cells, dendritic cells, type II great alveolar cells, and endothelial cells. IL-8 bioactivity is mediated through two G-protein-coupled receptors, termed CXCR1/IL-8 RA and CXCR2/IL-8 RB. CXCR1 is 45-50 kDa in size and is used almost exclusively by IL-8. CXCR2 is 35-40 kDa in size and is used by nearly all CXC chemokines. Both CXCR1 and CXCR2 constitutively associate into functional homodimers. They can also heterodimerize, but these complexes dissociate following IL-8 binding. CXCR2 responds to low concentrations of IL-8 and is principally associated with chemotaxis and MMP-9 release. CXCR1, in contrast, responds to high concentrations of IL-8 and is associated with respiratory burst and phospholipase D2 activation. Thus, CXCR2 ligation induces leukocyte adhesion to activated vascular endothelium and migration to sites of inflammation, while CXCR1 ligation primes neutrophil antimicrobial activity. IL-8 can also form a complex with Serpin A1/alpha-1 Antitrypsin, and this prevents IL-8 interaction with CXCR1. In addition to its pro-inflammatory effects, IL-8 is involved in angiogenesis and the pathogenesis of atherosclerosis and cancer. It induces VEGF expression in vascular endothelial cells and functions as an autocrine factor for EC growth and angiogenesis. It is upregulated in atherosclerotic lesions and is elevated in the serum and cerebrospinal fluid following myocardial infarction. In cancer, IL-8 promotes epithelial-mesenchymal transition as well as tumor cell invasiveness and metastasis.

文献・引用論文

アプリケーションで絞り込む

種属で絞り込む

IL-8/CXCR2 mediates resistance to apatinib through PI3K/AKT and vasculogenic mimicry in gastric cancer cells
- ジャーナル: Tissue & Cell
- DOI: 10.1016/j.tice.2025.103185
- IF: 2.5
- アプリケーション: ELISA
- 交差反応性: Human
- 掲載日: 2025 Oct
Investigating the Role of Quercetin, an Active Ingredient in Bazhen Decoction, in Targeting CXCL8 to Inhibit Macrophage M2 Polarization and Reshape the Immunological Microenvironment of Colorectal Cancer
- ジャーナル: Chemical Biology & Drug Design
- DOI:
- IF: 3.2
- アプリケーション: ELISA
- 交差反応性: Human
- 掲載日: 2025 Jan
Investigating the Role of Quercetin, an Active Ingredient in Bazhen Decoction, in Targeting CXCL8 to Inhibit Macrophage M2 Polarization and Reshape the Immunological Microenvironment of Colorectal Cancer
- ジャーナル: Chemical Biology & Drug Design
- DOI: 10.1111/cbdd.70047
- IF: 3.3
- アプリケーション: ELISA
- 交差反応性: Human
- 掲載日: 2025 Jan
Paris polyphylla var. yunnanensis Leaf-Derived Extracellular Vesicle-Like Particles Enhance Periodontal Regeneration
- ジャーナル: Biomaterials Research
- DOI: 10.34133/bmr.0291
- IF: 9.6
- アプリケーション: ELISA
- 交差反応性: Human
- 掲載日: 2025 Dec

Human IL-8/CXCL8 enzyme-linked immunoassay kit

お問い合わせ

製品概要

製品名

製品タイプ

検出感度

精度

スパイク回収

サンプル値

実験タイプ

種属反応性

検出方法

所要時間

標準曲線梯度

インキュベーション回数

Detectable Sample

サンプル添加量

製品の特徴

保存条件

文献におけるアプリケーション

文献におけるアプリケーション

ターゲット

UNIPROT #

別名

機能

文献・引用論文

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