Human Apolipoprotein E/Apo E enzyme-linked immunoassay kit(one step)
Catalog# EHY0237
Human Apolipoprotein E/Apo E enzyme-linked immunoassay kit(one step)
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ELISA
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Human
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15.63~1000 ng/ml
製品概要
製品名
Human Apolipoprotein E/Apo E enzyme-linked immunoassay kit(one step)
製品タイプ
Elisa Kit
検出感度
0.46 ng/ml (10 μl)
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0187 | 0.0194 | 0.0191 | |
| 15.63 | 0.0974 | 0.0943 | 0.0959 | 0.0768 |
| 31.25 | 0.1995 | 0.1820 | 0.1908 | 0.1717 |
| 62.50 | 0.4094 | 0.3758 | 0.3926 | 0.3736 |
| 125.00 | 0.8391 | 0.8584 | 0.8488 | 0.8297 |
| 250.00 | 1.6260 | 1.5950 | 1.6105 | 1.5915 |
| 500.00 | 3.1190 | 3.0560 | 3.0875 | 3.0685 |
| 1000.00 | 4.3897 | 4.3803 | 4.3850 | 4.3660 |
精度
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (ng/ml) | 20.9 | 124.5 | 383.8 | 20.0 | 136.7 | 398.5 |
| Standard Deviation | 1.6 | 9.3 | 27.8 | 1.1 | 6.8 | 9.7 |
| Coefficient of Variation (%) | 7.6 | 7.5 | 7.3 | 5.5 | 5.0 | 2.4 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
スパイク回収
The spike recovery was evaluated by spiking 3 levels of human Apolipoprotein E/Apo E into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 119% with an overall mean recovery of 103%.
サンプル値
| Sample Matrix | Sample Evaluated | Range (μg/ml) | Detectable (%) | Mean of Detectable (μg/ml) |
|---|---|---|---|---|
| Serum | 30 | 23.36-55.33 | 100 | 37.04 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated in this assay. No medical histories were available for the donors.
実験タイプ
種属反応性
検出方法
所要時間
標準曲線梯度
インキュベーション回数
2
Detectable Sample
サンプル添加量
製品の特徴
保存条件
2-8 ℃で保存