Human CX3CL1/Fractalking enzyme-linked immunoassay kit
Catalog# EH0213
Human CX3CL1/Fractalking enzyme-linked immunoassay kit
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ELISA
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Human
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156.25~10000 pg/ml
製品概要
製品名
Human CX3CL1/Fractalking enzyme-linked immunoassay kit
製品タイプ
Elisa Kit
検出感度
7.47 pg/ml (50 μl)；16.03 pg/ml (10 μl)；
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0138 | 0.0133 | 0.0136 | |
| 156.25 | 0.0427 | 0.0364 | 0.0396 | 0.0260 |
| 312.50 | 0.0842 | 0.0711 | 0.0777 | 0.0641 |
| 625.00 | 0.1921 | 0.1773 | 0.1847 | 0.1712 |
| 1250.00 | 0.5697 | 0.4696 | 0.5197 | 0.5061 |
| 2500.00 | 1.3220 | 1.2500 | 1.2860 | 1.2725 |
| 5000.00 | 2.5240 | 2.4520 | 2.4880 | 2.4745 |
| 10000.00 | 3.5110 | 3.5180 | 3.5145 | 3.5010 |
精度
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 285.8 | 1505.4 | 4447.7 | 294.6 | 1388.2 | 4357.8 |
| Standard Deviation | 21.7 | 80.1 | 223.3 | 12.3 | 74.3 | 153.6 |
| Coefficient of Variation (%) | 7.6 | 5.3 | 5.0 | 4.2 | 5.4 | 3.5 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
スパイク回収
The spike recovery was evaluated by spiking 3 levels of Human CX3CL1/Fractalking into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 79% to 128% with an overall mean recovery of 114%.
サンプル値
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 516.05-2692.08 | 100 | 1625.88 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of CX3CL1/Fractalking in this assay. No medical histories were available for the donors.
実験タイプ
種属反応性
検出方法
所要時間
標準曲線梯度
インキュベーション回数
2
Detectable Sample
サンプル添加量
製品の特徴
保存条件
2-8 ℃で保存
ターゲット
UNIPROT #
別名
A 152E5.2
AB030188
ABCD 3
ABCD3
AI848747
C-X3-C motif chemokine 1
C3Xkine
Chemokine (C-X3-C motif) ligand 1
Chemokine C X3 C motif ligand 1
Chemokine CX3C Motif Ligand 1
展開