Human KIM-1/HAVCR1/TIM-1 enzyme-linked immunoassay kit
Catalog# EH0241
Human KIM-1/HAVCR1/TIM-1 enzyme-linked immunoassay kit
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ELISA
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Human
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1.37~1000 pg/ml
製品概要
製品名
Human KIM-1/HAVCR1/TIM-1 enzyme-linked immunoassay kit
製品タイプ
Elisa Kit
検出感度
0.01 pg/ml (50 μl)；2.87 pg/ml (10 μl)；
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0108 | 0.0102 | 0.0105 | |
| 1.37 | 0.0178 | 0.0167 | 0.0173 | 0.0068 |
| 4.12 | 0.0284 | 0.0298 | 0.0291 | 0.0186 |
| 12.35 | 0.0646 | 0.0667 | 0.0657 | 0.0552 |
| 37.04 | 0.1792 | 0.1831 | 0.1812 | 0.1707 |
| 111.11 | 0.4724 | 0.4993 | 0.4859 | 0.4754 |
| 333.33 | 1.3460 | 1.3870 | 1.3665 | 1.3560 |
| 1000.00 | 3.3360 | 3.3650 | 3.3505 | 3.3400 |
精度
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 14.1 | 103.9 | 401.6 | 16.1 | 102.7 | 314.1 |
| Standard Deviation | 1.0 | 6.9 | 30.6 | 0.7 | 2.9 | 14.4 |
| Coefficient of Variation (%) | 7.1 | 6.6 | 7.6 | 4.1 | 2.9 | 4.6 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
スパイク回収
The spike recovery was evaluated by spiking 3 levels of human KIM-1/HAVCR1/TIM-1 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 82% to 125% with an overall mean recovery of 111%.
サンプル値
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 7.08-28.75 | 100 | 14.61 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated in this assay. No medical histories were available for the donors.
実験タイプ
種属反応性
検出方法
所要時間
標準曲線梯度
インキュベーション回数
2
Detectable Sample
サンプル添加量
製品の特徴
保存条件
2-8 ℃で保存