Human TREM2 enzyme-linked immunoassay kit（one step）

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Catalog# EHY0122

Human TREM2 enzyme-linked immunoassay kit（one step）

Application

ELISA

Reactivity

Human

Standard curve range

0.14~100 ng/ml

製品概要

製品名

Human TREM2 enzyme-linked immunoassay kit（one step）

製品タイプ

Elisa Kit

検出感度

0.004 ng/ml (50 μl)；0.05 ng/ml (50 μl)

ng/ml	O.D.		Average	Corrected
0.00	0.0061	0.0054	0.0058
0.14	0.0116	0.0115	0.0116	0.0058
0.41	0.0267	0.0281	0.0274	0.0217
1.23	0.0789	0.0792	0.0791	0.0733
3.70	0.2296	0.2361	0.2329	0.2271
11.11	0.6794	0.6593	0.6694	0.6636
33.33	1.7740	1.7290	1.7515	1.7458
100.00	3.3700	3.3340	3.3520	3.3463

精度

	Intra-Assay Precision			Inter-Assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average (ng/ml)	1.9	10.3	35.3	2.0	10.0	32.2
Standard Deviation	0.1	0.4	1.8	0.1	0.5	1.9
Coefficient of Variation (%)	2.8	3.6	5.1	4.1	4.9	5.9

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

スパイク回収

The spike recovery was evaluated by spiking 3 levels of Human TREM2 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 95% to 105% with an overall mean recovery of 101%.

サンプル値

Sample Matrix	Sample Evaluated	Range (ng/ml)	Detectable (%)	Mean of Detectable (ng/ml)
Serum	30	1.20-4.84	100.0	2.69

Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of TREM2 in this assay. No medical histories were available for the donors.

実験タイプ

Sandwich Elisa

種属反応性

Human

検出方法

Colorimetric

所要時間

60min

標準曲線梯度

7点/3倍

インキュベーション回数

Detectable Sample

血清，血浆

サンプル添加量

50 μl/10 μl

製品の特徴

保存条件

2-8 ℃で保存

ターゲット

UNIPROT #

SwissProt: Q9NZC2 Human

別名

TREM 2

TREM-2

TREM2

TREM2_HUMAN

TREM2a

TREM2b

TREM2c

Trggering receptor expressed on myeloid cells 2

Trggering receptor expressed on myeloid cells 2a

Triggering receptor expressed on monocytes 2

展開

機能

TREM2 (Triggering Receptor Expressed by Myeloid cells) is an Ig superfamily cell surface receptor that activates a number of myeloid cell types. It is a member of a small gene family located on human chromosome 6p21 and mouse chromosome 17 in a region linked to the MHC. A single human TREM2 gene has been described, however, two closely related orthologs were reported in mouse. The proteins differ by only three amino acids and were designated TREM2a and TREM2b. TREM2 is type I transmembrane protein consisting of a single extracellular immunoglobulin (V-like) domain, a transmembrane domain with a positively charged lysine residue, and a short cytoplasmic tail. It associates with the signal adapter protein, DAP12, for signaling and function. DAP12 has a cytoplasmic ITAM that is phosphorylated upon ligand binding leading to the subsequent activation of cytoplasmic tyrosine kinases. TREM2 is expressed by immature monocyte-derived dendritic cells (DC), and expression is down-regulated upon activation of DC by microbial products and costimulatory signals. Ligation of TREM2 on immature DC with anti-TREM2 antibodies results in partial DC activation and the up-regulation of CCR7 and some co-stimulatory molecules. A role for TREM2 in the functioning of osteoclasts and microglia is suggested by the discovery that homozygous loss-of-function mutations in either TREM2 or DAP12 result in Nasu-Hakola disease characterized by a combination of presenile demetia and bone cysts. In vitro studies indicate that the differentiation of myeloid precursors into osteoclasts is dramatically impaired in TREM2 deficient individuals.

Human TREM2 enzyme-linked immunoassay kit（one step）

お問い合わせ

製品概要

製品名

製品タイプ

検出感度

精度

スパイク回収

サンプル値

実験タイプ

種属反応性

検出方法

所要時間

標準曲線梯度

インキュベーション回数

Detectable Sample

サンプル添加量

製品の特徴

保存条件

ターゲット

UNIPROT #

別名

機能

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