Human Acetylcholinesterase/ACHE enzyme-linked immunoassay kit
Catalog# EH0226
Human Acetylcholinesterase/ACHE enzyme-linked immunoassay kit
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ELISA
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Human
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40.96~10000 pg/ml
製品概要
製品名
Human Acetylcholinesterase/ACHE enzyme-linked immunoassay kit
製品タイプ
Elisa Kit
検出感度
0.85 pg/ml (50 μl)；8.61 pg/ml (10 μl)；
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0099 | 0.0094 | 0.0097 | |
| 40.96 | 0.0568 | 0.0541 | 0.0555 | 0.0458 |
| 102.40 | 0.1282 | 0.1271 | 0.1277 | 0.1180 |
| 256.00 | 0.3135 | 0.2983 | 0.3059 | 0.2963 |
| 640.00 | 0.7399 | 0.6988 | 0.7194 | 0.7097 |
| 1600.00 | 1.6520 | 1.6190 | 1.6355 | 1.6259 |
| 4000.00 | 3.1140 | 2.9580 | 3.0360 | 3.0264 |
| 10000.00 | 4.3172 | 4.2587 | 4.2880 | 4.2783 |
精度
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 174.5 | 844.4 | 2868.9 | 182.3 | 847.9 | 2927.1 |
| Standard Deviation | 3.7 | 23.5 | 108.7 | 9.0 | 40.8 | 78.6 |
| Coefficient of Variation (%) | 2.1 | 2.8 | 3.8 | 4.9 | 4.8 | 2.7 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
スパイク回収
The spike recovery was evaluated by spiking 3 levels of human Acetylcholinesterase/ACHE into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 82% to 120% with an overall mean recovery of 99%.
サンプル値
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 9.04-19.15 | 100 | 12.18 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated in this assay. No medical histories were available for the donors.
実験タイプ
種属反応性
検出方法
所要時間
標準曲線梯度
インキュベーション回数
2
Detectable Sample
サンプル添加量
製品の特徴
保存条件
2-8 ℃で保存