Human CD63 enzyme-linked immunoassay kit
Catalog# EH0167
Human CD63 enzyme-linked immunoassay kit
-
ELISA
-
Human
-
6.86~5000 pg/ml
製品概要
製品名
Human CD63 enzyme-linked immunoassay kit
製品タイプ
Elisa Kit
検出感度
0.24 pg/ml (50 μl)；6.71 pg/ml (10 μl)；
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0151 | 0.0128 | 0.0140 | |
| 6.86 | 0.0286 | 0.0280 | 0.0283 | 0.0144 |
| 20.58 | 0.0572 | 0.0582 | 0.0577 | 0.0438 |
| 61.73 | 0.1475 | 0.1458 | 0.1467 | 0.1327 |
| 185.19 | 0.4194 | 0.3984 | 0.4089 | 0.3950 |
| 555.56 | 1.2120 | 1.1120 | 1.1620 | 1.1481 |
| 1666.67 | 2.6950 | 2.5740 | 2.6345 | 2.6206 |
| 5000.00 | 3.8760 | 3.8910 | 3.8835 | 3.8696 |
精度
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 336.6 | 1807.6 | 5114.7 | 325.3 | 1844.1 | 5419.5 |
| Standard Deviation | 10.2 | 76.5 | 268.5 | 11.8 | 98.5 | 124.0 |
| Coefficient of Variation (%) | 3.0 | 4.2 | 5.2 | 3.6 | 5.3 | 2.3 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
スパイク回収
The spike recovery was evaluated by spiking 3 levels of human CD63 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 93% to 119% with an overall mean recovery of 105%.
サンプル値
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 570.74-1262.25 | 100 | 787.53 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of CD63 in this assay. No medical histories were available for the donors.
実験タイプ
種属反応性
検出方法
所要時間
標準曲線梯度
インキュベーション回数
2
Detectable Sample
サンプル添加量
製品の特徴
保存条件
2-8 ℃で保存
ターゲット
UNIPROT #
別名
Lysosomal associated membrane protein 3
CD 63
CD63
CD63 antigen (melanoma 1 antigen)
CD63 antigen
CD63 antigen melanoma 1 antigen
CD63 molecule
CD63_HUMAN
gp55
Granulophysin
展開