Human CCL3/MIP-1 enzyme-linked immunoassay kit
Catalog# EH0146
Human CCL3/MIP-1 enzyme-linked immunoassay kit
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ELISA
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Human
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2.74~2000 pg/ml
製品概要
製品名
Human CCL3/MIP-1 enzyme-linked immunoassay kit
製品タイプ
Elisa Kit
検出感度
0.05 pg/ml (50 μl)；0.52 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0167 | 0.0161 | 0.0164 | |
| 2.74 | 0.0479 | 0.0428 | 0.0454 | 0.0290 |
| 8.23 | 0.0873 | 0.0832 | 0.0853 | 0.0689 |
| 24.69 | 0.2058 | 0.2010 | 0.2034 | 0.1870 |
| 74.07 | 0.6641 | 0.5979 | 0.6310 | 0.6146 |
| 222.22 | 1.4760 | 1.4170 | 1.4465 | 1.4301 |
| 666.67 | 2.5390 | 2.5340 | 2.5365 | 2.5201 |
| 2000.00 | 3.5360 | 3.5510 | 3.5435 | 3.5271 |
精度
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 55.8 | 271.9 | 819.4 | 33.7 | 173.5 | 578.5 |
| Standard Deviation | 2.6 | 17.2 | 28.5 | 2.0 | 13.4 | 42.3 |
| Coefficient of Variation (%) | 4.7 | 6.3 | 3.5 | 6.0 | 7.7 | 7.3 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
スパイク回収
The spike recovery was evaluated by spiking 3 levels of human CCL3/MIP-1 alpha into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 94% to 114% with an overall mean recovery of 102%.
サンプル値
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 12.28-1051.89 | 100 | 224.55 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of CCL3/MIP-1 in this assay. No medical histories were available for the donors.
実験タイプ
種属反応性
検出方法
所要時間
標準曲線梯度
インキュベーション回数
2
Detectable Sample
サンプル添加量
製品の特徴
保存条件
2-8 ℃で保存
ターゲット
UNIPROT #
別名
C C motif chemokine 3
CCL 3
CCL3
CCL3_HUMAN
Chemokine C C motif ligand 3
G0/G1 switch regulatory protein 19 1
G0/G1 switch regulatory protein 19-1
G0S19 1
G0S19 1 protein
Heparin binding chemotaxis protein
展開