Human Pentraxin 3/PTX3 enzyme-linked immunoassay kit
Catalog# EH0141
Human Pentraxin 3/PTX3 enzyme-linked immunoassay kit
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ELISA
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Human
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13.73~10000 pg/ml
製品概要
製品名
Human Pentraxin 3/PTX3 enzyme-linked immunoassay kit
製品タイプ
Elisa Kit
検出感度
1.99 pg/ml (50 μl)；9.53 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0431 | 0.0393 | 0.0412 | |
| 13.72 | 0.0704 | 0.0717 | 0.0711 | 0.0299 |
| 41.15 | 0.1370 | 0.1360 | 0.1365 | 0.0953 |
| 123.46 | 0.3247 | 0.3126 | 0.3187 | 0.2775 |
| 370.37 | 0.8276 | 0.7558 | 0.7917 | 0.7505 |
| 1111.11 | 1.7600 | 1.6140 | 1.6870 | 1.6458 |
| 3333.33 | 3.0380 | 2.8520 | 2.9450 | 2.9038 |
| 10000.00 | 3.8390 | 3.6580 | 3.7485 | 3.7073 |
精度
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 213.5 | 1031.5 | 3290.1 | 242.9 | 1110.6 | 3753.9 |
| Standard Deviation | 11.3 | 63.9 | 223.3 | 5.3 | 51.7 | 224.3 |
| Coefficient of Variation (%) | 5.3 | 6.2 | 6.8 | 2.2 | 4.7 | 6.0 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
スパイク回収
The spike recovery was evaluated by spiking 3 levels of human Pentraxin 3/PTX3 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 118% with an overall mean recovery of 104%.
サンプル値
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 1308.85-6892.74 | 100 | 3254.3 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of Pentraxin 3/PTX3 in this assay. No medical histories were available for the donors.
実験タイプ
種属反応性
検出方法
所要時間
標準曲線梯度
インキュベーション回数
2
Detectable Sample
サンプル添加量
製品の特徴
保存条件
2-8 ℃で保存
ターゲット
UNIPROT #
別名
AI607804
Pentaxin 3
Pentaxin-related protein PTX3
Pentraxin 3 long
Pentraxin-related gene
Pentraxin-related gene rapidly induced by IL-1 beta
Pentraxin-related protein PTX3
PTX3
PTX3_HUMAN
TNF alpha-induced protein 5
展開