Human TIM4 enzyme-linked immunoassay kit(one step)
Catalog# EHY0194
Human TIM4 enzyme-linked immunoassay kit(one step)
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ELISA
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Human
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0.14~100 ng/ml
製品概要
製品名
Human TIM4 enzyme-linked immunoassay kit(one step)
製品タイプ
Elisa Kit
検出感度
0.001 ng/ml (50 μl)；0.03 ng/ml (10 μl)；
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0066 | 0.0066 | 0.0066 | |
| 0.14 | 0.0584 | 0.0587 | 0.0586 | 0.0520 |
| 0.41 | 0.1645 | 0.1618 | 0.1632 | 0.1566 |
| 1.23 | 0.4574 | 0.4633 | 0.4604 | 0.4538 |
| 3.70 | 1.2320 | 1.2350 | 1.2335 | 1.2269 |
| 11.11 | 2.6720 | 2.6900 | 2.6810 | 2.6744 |
| 33.33 | 3.4720 | 3.4610 | 3.4665 | 3.4599 |
| 100.00 | 3.7210 | 3.6020 | 3.6615 | 3.6549 |
精度
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (ng/ml) | 1.7 | 12.8 | 30.6 | 1.6 | 13.8 | 31.6 |
| Standard Deviation | 0.1 | 1.1 | 4.1 | 0.1 | 1.5 | 5.2 |
| Coefficient of Variation (%) | 3.1 | 4.5 | 3.2 | 2.7 | 3.9 | 4.2 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
スパイク回収
The spike recovery was evaluated by spiking 3 levels of human TIM4 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 89% to 113% with an overall mean recovery of 98%.
サンプル値
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 4.94-10.86 | 100 | 7.18 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of TIM4 in this assay. No medical histories were available for the donors.
実験タイプ
種属反応性
検出方法
所要時間
標準曲線梯度
インキュベーション回数
2
Detectable Sample
サンプル添加量
製品の特徴
保存条件
2-8 ℃で保存
ターゲット
UNIPROT #
別名
SMUCKLER
Spleen
mucin-containing
knockout of lymphotoxin protein
T cell immunoglobulin and mucin domain containing protein 4
T-cell immunoglobulin and mucin domain containing 4
T-cell immunoglobulin and mucin domain containing molecule
T-cell immunoglobulin and mucin domain-containing protein 4
T-cell immunoglobulin and mucin domains-containing protein 4
T-cell membrane protein 4
展開