Human CXCL13/BCA-1 enzyme-linked immunoassay kit
Catalog# EH0172
Human CXCL13/BCA-1 enzyme-linked immunoassay kit
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ELISA
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Human
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7.81~500 pg/ml
製品概要
製品名
Human CXCL13/BCA-1 enzyme-linked immunoassay kit
製品タイプ
Elisa Kit
検出感度
0.56 pg/ml (50 μl)；5.32 pg/ml (10 μl)；
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0076 | 0.0074 | 0.0075 | |
| 7.81 | 0.0260 | 0.0265 | 0.0263 | 0.0188 |
| 15.63 | 0.0515 | 0.0509 | 0.0512 | 0.0437 |
| 31.25 | 0.1190 | 0.1180 | 0.1185 | 0.1110 |
| 62.50 | 0.2836 | 0.2700 | 0.2768 | 0.2693 |
| 125.00 | 0.7076 | 0.6990 | 0.7033 | 0.6958 |
| 250.00 | 1.8240 | 1.8140 | 1.8190 | 1.8115 |
| 500.00 | 3.5660 | 3.6350 | 3.6005 | 3.5930 |
精度
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 19.3 | 78.3 | 213.9 | 18.5 | 76.1 | 216.7 |
| Standard Deviation | 0.7 | 2.7 | 9.6 | 0.7 | 2.3 | 7.1 |
| Coefficient of Variation (%) | 3.4 | 3.4 | 4.5 | 3.8 | 3.0 | 3.3 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
スパイク回収
The spike recovery was evaluated by spiking 3 levels of human CXCL13/BCA-1 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 120% with an overall mean recovery of 96%.
サンプル値
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 28.44-275.19 | 100 | 71.8 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of CXCL13/BCA-1 in this assay. No medical histories were available for the donors.
実験タイプ
種属反応性
検出方法
所要時間
標準曲線梯度
インキュベーション回数
2
Detectable Sample
サンプル添加量
製品の特徴
保存条件
2-8 ℃で保存
ターゲット
UNIPROT #
別名
ANGIE
ANGIE2
B cell attracting chemokine 1
B cell-attracting chemokine 1
B lymphocyte chemoattractant
B-cell chemoattractant
B-cell-attracting chemokine 1
B-cell-homing chemokine (ligand for Burkitt's lymphoma receptor-1)
BCA-1
BCA1
展開