Human FABP2 enzyme-linked immunoassay kit
Catalog# EH0256
Human FABP2 enzyme-linked immunoassay kit
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ELISA
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Human
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10.97~8000 pg/ml
製品概要
製品名
Human FABP2 enzyme-linked immunoassay kit
製品タイプ
Elisa Kit
検出感度
1.85 pg/ml (50 μl)；9.48 pg/ml (10 μl)；
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0127 | 0.0117 | 0.0122 | |
| 10.97 | 0.0221 | 0.0197 | 0.0209 | 0.0087 |
| 32.92 | 0.0360 | 0.0352 | 0.0356 | 0.0234 |
| 98.77 | 0.0823 | 0.0774 | 0.0799 | 0.0677 |
| 296.30 | 0.2234 | 0.2171 | 0.2203 | 0.2081 |
| 888.89 | 0.6406 | 0.6136 | 0.6271 | 0.6149 |
| 2666.67 | 1.7600 | 1.7220 | 1.7410 | 1.7288 |
| 8000.00 | 3.7530 | 3.8030 | 3.7780 | 3.7658 |
精度
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 2676.88 | 824.36 | 163.87 | 2575.6 | 777.7 | 160.5 |
| Standard Deviation | 151.57 | 35.86 | 10.04 | 60.3 | 14.0 | 7.1 |
| Coefficient of Variation (%) | 5.7 | 4.4 | 6.1 | 2.3 | 2.3 | 3.2 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
スパイク回収
The spike recovery was evaluated by spiking 3 levels of human FABP2 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 81% to 115% with an overall mean recovery of 101%.
サンプル値
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 517.21-2083.92 | 100 | 1262.00 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated in this assay. No medical histories were available for the donors.
実験タイプ
種属反応性
検出方法
所要時間
標準曲線梯度
インキュベーション回数
2
Detectable Sample
サンプル添加量
製品の特徴
保存条件
2-8 ℃で保存