Human Galectin-9/LGALS9 enzyme-linked immunoassay kit
Catalog# EH0315
Human Galectin-9/LGALS9 enzyme-linked immunoassay kit
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sELISA
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Human
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13.72 ~10000 pg/ml
製品概要
製品名
Human Galectin-9/LGALS9 enzyme-linked immunoassay kit
製品タイプ
Elisa Kit
検出感度
1.38pg/ml (50 μl)；5.95pg/ml (10 μl)；
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0066 | 0.0059 | 0.0063 | |
| 13.72 | 0.0180 | 0.0140 | 0.0160 | 0.0098 |
| 41.15 | 0.0372 | 0.0314 | 0.0343 | 0.0281 |
| 123.46 | 0.0982 | 0.0935 | 0.0959 | 0.0896 |
| 370.37 | 0.2808 | 0.2398 | 0.2603 | 0.2541 |
| 1111.11 | 0.7660 | 0.6679 | 0.7170 | 0.7107 |
| 3333.33 | 1.9190 | 1.8850 | 1.9020 | 1.8958 |
| 10000.00 | 3.9880 | 3.9520 | 3.9700 | 3.9638 |
精度
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 149.0 | 818.1 | 2827.2 | 157.3 | 855.1 | 3030.7 |
| Standard Deviation | 8.4 | 34.3 | 198.8 | 8.5 | 29.7 | 111.4 |
| Coefficient of Variation (%) | 5.6 | 4.2 | 7.0 | 5.4 | 3.5 | 3.7 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
スパイク回収
The spike recovery was evaluated by spiking 3 levels of human Galectin-9/LGALS9 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 85% to 114% with an overall mean recovery of 105%.
サンプル値
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 5.86-22.04 | 100.0 | 12.35 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of human Galectin-9/LGALS9 in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
実験タイプ
種属反応性
検出方法
所要時間
標準曲線梯度
インキュベーション回数
2
Detectable Sample
サンプル添加量
製品の特徴
保存条件
2-8 ℃で保存
ターゲット
UNIPROT #
別名
Galectin-9
Gal-9
Ecalectin
Tumor antigen HOM-HD-21
LGALS9