Human CTLA-4 enzyme-linked immunoassay kit
Catalog# EH0179
Human CTLA-4 enzyme-linked immunoassay kit
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ELISA
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Human
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2.74~2000 pg/ml
製品概要
製品名
Human CTLA-4 enzyme-linked immunoassay kit
製品タイプ
Elisa Kit
検出感度
1.33 pg/ml (50 μl)；1.97 pg/ml (10 μl)；
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0105 | 0.0103 | 0.0104 | |
| 2.74 | 0.0171 | 0.0159 | 0.0165 | 0.0061 |
| 8.23 | 0.0282 | 0.0271 | 0.0277 | 0.0173 |
| 24.69 | 0.0616 | 0.0611 | 0.0614 | 0.0510 |
| 74.07 | 0.1683 | 0.1480 | 0.1582 | 0.1478 |
| 222.22 | 0.5143 | 0.4790 | 0.4967 | 0.4863 |
| 666.67 | 1.5540 | 1.5790 | 1.5665 | 1.5561 |
| 2000.00 | 3.9590 | 3.9100 | 3.9345 | 3.9241 |
精度
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 53.1 | 266.2 | 814.9 | 17.2 | 77.5 | 231.4 |
| Standard Deviation | 2.9 | 9.1 | 34.8 | 1.3 | 5.1 | 12.0 |
| Coefficient of Variation (%) | 5.5 | 3.4 | 4.3 | 7.8 | 6.6 | 5.2 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
スパイク回収
The spike recovery was evaluated by spiking 3 levels of human CTLA-4 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 91% to 97% with an overall mean recovery of 94%.
サンプル値
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 0.31-3.78 | 100 | 1.11 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of CTLA-4 in this assay. No medical histories were available for the donors.
実験タイプ
種属反応性
検出方法
所要時間
標準曲線梯度
インキュベーション回数
2
Detectable Sample
サンプル添加量
製品の特徴
保存条件
2-8 ℃で保存