Human IL-17A enzyme-linked immunoassay kit
Catalog# EH0004
Human IL-17A enzyme-linked immunoassay kit
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ELISA
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Human
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1.37~1000 pg/ml
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unconjugated
製品概要
製品名
Human IL-17A enzyme-linked immunoassay kit
製品タイプ
Elisa Kit
検出感度
0.26 pg/ml (50 μl) ；0.65 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0 | 0.0219 | 0.0209 | 0.0214 | |
| 1.37 | 0.0261 | 0.0261 | 0.0261 | 0.0047 |
| 4.12 | 0.0379 | 0.0395 | 0.0387 | 0.0173 |
| 12.35 | 0.0771 | 0.085 | 0.0811 | 0.0597 |
| 37.04 | 0.1799 | 0.1733 | 0.1766 | 0.1552 |
| 111.11 | 0.5178 | 0.5259 | 0.5219 | 0.5005 |
| 333.33 | 1.3929 | 1.4109 | 1.4019 | 1.3805 |
| 1000 | 3.2888 | 3.2794 | 3.2841 | 3.2627 |
精度
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 27.6 | 113.6 | 265.5 | 23.9 | 125.5 | 324.1 |
| Standard Deviation | 0.9 | 5.3 | 11.6 | 0.8 | 4.2 | 8.2 |
| Coefficient of Variation (%) | 3.2 | 4.6 | 4.4 | 3.3 | 3.3 | 2.5 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
スパイク回収
The spike recovery was evaluated by spiking 3 levels of human IL-17A into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 112% with an overall mean recovery of 96%.
サンプル値
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-10.72 | 40 | 2.03 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of IL-10 in this assay. No medical histories were available for the donors.
n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
実験タイプ
種属反応性
検出方法
所要時間
標準曲線梯度
インキュベーション回数
2
Detectable Sample
サンプル添加量
製品の特徴
保存条件
2-8 ℃で保存
ターゲット
UNIPROT #
別名
CTLA 8
CTLA-8
CTLA8
cytotoxic T lymphocyte associated antigen 8
Cytotoxic T lymphocyte associated protein 8
Cytotoxic T lymphocyte associated serine esterase 8
Cytotoxic T-lymphocyte-associated antigen 8
IL 17
IL 17A
IL-17
展開