Human ULBP1/NKG2DL1 enzyme-linked immunoassay kit

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Catalog# EH0150

Human ULBP1/NKG2DL1 enzyme-linked immunoassay kit

Application

ELISA

Reactivity

Human

Standard curve range

1.37~1000 pg/ml

製品概要

製品名

Human ULBP1/NKG2DL1 enzyme-linked immunoassay kit

製品タイプ

Elisa Kit

検出感度

0.06 pg/ml (50 μl)；0.87 pg/ml (10 μl)

pg/ml	O.D.		Average	Corrected
0.00	0.0193	0.0180	0.0187
1.37	0.0348	0.0335	0.0342	0.0155
4.12	0.0687	0.0626	0.0657	0.0470
12.35	0.1540	0.1598	0.1569	0.1383
37.04	0.4233	0.3886	0.4060	0.3873
111.11	1.1080	1.0290	1.0685	1.0499
333.33	2.4510	2.4130	2.4320	2.4134
1000.00	3.8060	3.8000	3.8030	3.7844

精度

	Intra-Assay Precision			Inter-Assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average (pg/ml)	16.4	85.0	258.5	17.5	92.7	302.1
Standard Deviation	0.7	2.3	14.5	0.7	3.9	15.9
Coefficient of Variation (%)	4.4	2.7	5.6	3.7	4.2	5.3

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

スパイク回収

The spike recovery was evaluated by spiking 3 levels of human ULBP1/NKG2DL1 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 82% to 120% with an overall mean recovery of 99%.

サンプル値

Sample Matrix	Sample Evaluated	Range (pg/ml)	Detectable (%)	Mean of Detectable (pg/ml)
Serum	30	0.18-1.88	100	0.8

Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of ULBP1/NKG2DL1 in this assay. No medical histories were available for the donors.

実験タイプ

Sandwich Elisa

種属反応性

Human

検出方法

Colorimetric

所要時間

120min

標準曲線梯度

7点/3倍

インキュベーション回数

Detectable Sample

可溶性物质的液相样品。例如：血清，血浆，细胞培养上清，组织研磨液等。

サンプル添加量

50 μl/10 μl

製品の特徴

保存条件

2-8 ℃で保存

ターゲット

UNIPROT #

SwissProt: Q9BZM6 Human

別名

UL16-binding protein 1、ALCAN-beta、NKG2D ligand 1、N2DL-1、NKG2DL1

機能

ULBP-1 is a member of a family of cell-surface proteins that function as ligands for human NKG2D. ULBP-1 has also been described under the names RaeT1I (retinoic acid early transcript), ALCAN-beta, and NKG2DL1. The name ULBP-1 derives from the original identification of three proteins, ULBP-1, -2, and -3, as ligands for the human cytomegalovirus glycoprotein UL16; they were designated UL16 binding proteins (ULBP). The gene for ULBP-1 resides in a cluster of ten related genes, six of which encode potentially functional glycoproteins. Amino acid sequence identity within this family ranges from 30-95%. These proteins are distantly related to MHC class I proteins, but they possess only the alpha 1 and alpha 2 Ig-like domains, and they have no capacity to bind peptide or interact with beta 2-microglobulin. They are anchored to the membrane via a GPI-linkage. ULBP-1 and several other family members are known to bind to human NKG2D, an activating receptor expressed on NK cells, NKT cells, gamma δ T cells, and CD8+ alpha beta T cells. Engagement of NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells. ULBP-1 is expressed on some tumor cells and has been implicated in tumor surveillance.

Human ULBP1/NKG2DL1 enzyme-linked immunoassay kit

お問い合わせ

製品概要

製品名

製品タイプ

検出感度

精度

スパイク回収

サンプル値

実験タイプ

種属反応性

検出方法

所要時間

標準曲線梯度

インキュベーション回数

Detectable Sample

サンプル添加量

製品の特徴

保存条件

ターゲット

UNIPROT #

別名

機能

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