Human CD30 enzyme-linked immunoassay kit

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Catalog# EH0105

Human CD30 enzyme-linked immunoassay kit

Application

ELISA

Reactivity

Human

Standard curve range

5.49~4000 pg/ml

製品概要

製品名

Human CD30 enzyme-linked immunoassay kit

製品タイプ

Elisa Kit

検出感度

0.8 pg/ml (50 μl)；3.19 pg/ml (10 μl)

pg/ml	O.D.		Average	Corrected
0.00	0.0199	0.0202	0.0201
5.49	0.0348	0.0353	0.0351	0.0150
16.46	0.0703	0.0685	0.0694	0.0494
49.38	0.1718	0.1691	0.1705	0.1504
148.15	0.4668	0.4436	0.4552	0.4352
444.44	1.2290	1.2290	1.2290	1.2090
1333.33	2.7640	2.8060	2.7850	2.7650
4000.00	4.3046	4.2674	4.2860	4.2660

精度

	Intra-Assay Precision			Inter-Assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average (pg/ml)	87.5	398.2	1088.4	84.1	408.2	1263.3
Standard Deviation	5.2	21.2	81.8	2.9	25.6	82.2
Coefficient of Variation (%)	6.0	5.3	6.0	3.5	6.3	6.5

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

スパイク回収

The spike recovery was evaluated by spiking 3 levels of human CD30 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 83% to 98% with an overall mean recovery of 93%.

サンプル値

Sample Matrix	Sample Evaluated	Range (pg/ml)	Detectable (%)	Mean of Detectable (pg/ml)
Serum	30	643.28-1550.80	100	965.76

Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of IL-12/IL-23 p40 in this assay. No medical histories were available for the donors.

実験タイプ

Sandwich Elisa

種属反応性

Human

検出方法

Colorimetric

所要時間

120min

標準曲線梯度

7点/3倍

インキュベーション回数

Detectable Sample

可溶性物质的液相样品。例如：血清，血浆，细胞培养上清，组织研磨液等。

サンプル添加量

50 μl/10 μl

製品の特徴

保存条件

2-8 ℃で保存

ターゲット

UNIPROT #

SwissProt: P28908 Human

別名

CD 30

CD30

CD30 antigen

CD30L receptor

Cytokine receptor CD30

D1S166E

KI 1

KI 1 antigen

Ki-1 antigen

KI1

展開

機能

CD30, also known as Ki-1 antigen and TNFRSF8, is a 120 kDa type I transmembrane glycoprotein belonging to the TNF receptor superfamily. Mature human CD30 consists of a 361 amino acid (aa) extracellular domain (ECD) with six cysteine-rich repeats, a 28 aa transmembrane segment, and a 188 aa cytoplasmic domain. In contrast, mouse and rat CD30 lack 90 aa of the ECD and contain only three cysteine-rich repeats. Within common regions of the ECD, human CD30 shares 53% and 49% aa sequence identity with mouse and rat CD30, respectively. Alternate splicing of human CD30 generates an isoform that includes only the C‑terminal 132 aa of the cytoplasmic domain. CD30 is normally expressed on antigen-stimulated Th cells and B cells. However, it is upregulated in Hodgkin’s disease (on Reed-Sternberg cells), other lymphomas, chronic inflammation, and autoimmunity. CD30 binds to CD30 Ligand/TNFSF8 which is expressed on activated Th cells, monocytes, granulocytes and medullary thymic epithelial cells. CD30 signaling costimulates antigen-induced Th0 and Th2 proliferation and cytokine secretion but favors a Th2-biased immune response. In the absence of antigenic stimulation, it can still induce T cell expression of IL-13. CD30 contributes to thymic negative selection by inducing the apoptotic cell death of CD4+CD8+ T cells. In B cells, CD30 ligation promotes cellular proliferation and antibody production in addition to the expression of CXCR4, CCL3, and CCL5. An 85-90 kDa soluble form of CD30 is shed from the cell surface by TACE-mediated cleavage. Soluble CD30 retains the ability to bind CD30 Ligand and functions as an inhibitor of normal CD30 signaling.

Human CD30 enzyme-linked immunoassay kit

お問い合わせ

製品概要

製品名

製品タイプ

検出感度

精度

スパイク回収

サンプル値

実験タイプ

種属反応性

検出方法

所要時間

標準曲線梯度

インキュベーション回数

Detectable Sample

サンプル添加量

製品の特徴

保存条件

ターゲット

UNIPROT #

別名

機能

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