Human PDGF-BB enzyme-linked immunoassay kit

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Catalog# EH0068

Human PDGF-BB enzyme-linked immunoassay kit

Application

ELISA

Reactivity

Human

Standard curve range

27.43~20000 pg/ml

製品概要

製品名

Human PDGF-BB enzyme-linked immunoassay kit

製品タイプ

Elisa Kit

検出感度

7.56 pg/ml (50 μl)；2.79 pg/ml (10 μl)

pg/ml	O.D.		Average	Corrected
0.00	0.0089	0.0079	0.0084
27.43	0.0225	0.0201	0.0213	0.0129
82.30	0.0578	0.0504	0.0541	0.0457
246.91	0.1331	0.1487	0.1409	0.1325
740.74	0.3914	0.3825	0.3870	0.3786
2222.22	0.8560	0.8562	0.8561	0.8477
6666.67	1.5790	1.4540	1.5165	1.5081
20000.00	3.1680	3.0260	3.0970	3.0886

精度

	Intra-Assay Precision			Inter-Assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average (pg/ml)	388.0	1692.3	5668.5	404.0	1977.6	5851.8
Standard Deviation	11.4	74.7	142.2	11.8	43.6	106.6
Coefficient of Variation (%)	6.0	4.4	5.3	5.8	4.5	3.7

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

スパイク回収

The spike recovery was evaluated by spiking 3 levels of human PDGF-BB into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 91% to 112% with an overall mean recovery of 103%.

サンプル値

Sample Matrix	Sample Evaluated	Range (pg/ml)	Detectable (%)	Mean of Detectable (pg/ml)
Serum	30	217.26-1712.20	100	608.29

Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of PDGF-BB in this assay. No medical histories were available for the donors.

実験タイプ

Sandwich Elisa

種属反応性

Human

検出方法

Colorimetric

所要時間

120min

標準曲線梯度

7点/3倍

インキュベーション回数

Detectable Sample

可溶性物质的液相样品。例如：血清，血浆，细胞培养上清，组织研磨液等。

サンプル添加量

50 μl/10 μl

製品の特徴

保存条件

2-8 ℃で保存

ターゲット

UNIPROT #

SwissProt: P01127 Human

別名

Becaplermin

c sis

FLJ12858

Oncogene SIS

PDGF 2

PDGF B chain

PDGF Beta

PDGF subunit B

PDGF-2

PDGF2

展開

機能

Platelet-derived growth factor (PDGF) was discovered as a major mitogenic factor present in serum but absent from plasma. It was found to be secreted from the alpha ‑granules of platelets activated during the coagulation of blood to form serum. Subsequent studies have demonstrated that PDGF is not one molecule but three, each a dimeric combination of two distinct but structurally related peptide chains designated A and B. The dimeric isoforms PDGF-AA, AB and BB are differentially expressed in various cell types and their effects are mediated through two distinct receptors, termed alpha and beta. Differences exist in isoform binding to each receptor. In general, PDGF isoforms are potent mitogens for connective tissue cells, including dermal fibroblasts, glial cells, arterial smooth muscle cells and some epithelial and endothelial cells. In addition to its activity as a mitogen, PDGF is chemotactic for fibroblasts, smooth muscle cells, neutrophils and mononuclear cells. Other reported activities for PDGF include stimulation of granule release by neutrophils and monocytes, facilitation of steroid synthesis by Leydig cells, stimulation of neutrophil phagocytosis, inhibition of natural killer (NK) cell activity, stimulation of collagen synthesis, modulation of thrombospondin expression and secretion, stimulation of collagenase activity and secretion, induction of contraction of rat aorta strips in vitro, and transient induction of T cell IL-2 secretion accompanied by a down-regulation of IL-4 and IFN-gamma production, temporary effects that may allow clonal expansion of antigen-activated B and T helper lymphocytes prior to differentiation. PDGF also appears to be ubiquitous in neurons throughout the CNS, where it is suggested to play an important role in neuron survival and regeneration, and in mediation of glial cell proliferation and differentiation.

Human PDGF-BB enzyme-linked immunoassay kit

お問い合わせ

製品概要

製品名

製品タイプ

検出感度

精度

スパイク回収

サンプル値

実験タイプ

種属反応性

検出方法

所要時間

標準曲線梯度

インキュベーション回数

Detectable Sample

サンプル添加量

製品の特徴

保存条件

ターゲット

UNIPROT #

別名

機能

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