Human CHIT1 enzyme-linked immunoassay kit
Catalog# EH0186
Human CHIT1 enzyme-linked immunoassay kit
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ELISA
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Human
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2.74~2000 pg/ml
製品概要
製品名
Human CHIT1 enzyme-linked immunoassay kit
製品タイプ
Elisa Kit
検出感度
0.22 pg/ml (50 μl)；0.49 pg/ml (10 μl)；
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0155 | 0.0136 | 0.0146 | |
| 2.74 | 0.0232 | 0.0218 | 0.0225 | 0.0080 |
| 8.23 | 0.0381 | 0.0399 | 0.0390 | 0.0245 |
| 24.69 | 0.1039 | 0.0986 | 0.1013 | 0.0867 |
| 74.07 | 0.2901 | 0.3065 | 0.2983 | 0.2838 |
| 222.22 | 0.8336 | 0.9318 | 0.8827 | 0.8682 |
| 666.67 | 2.3258 | 2.3320 | 2.3289 | 2.3144 |
| 2000.00 | 4.0844 | 4.0230 | 4.0537 | 4.0392 |
精度
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 47.01 | 199.00 | 780.94 | 46.20 | 201.75 | 671.27 |
| Standard Deviation | 1.72 | 9.54 | 45.28 | 1.93 | 5.46 | 38.00 |
| Coefficient of Variation (%) | 3.7 | 4.8 | 5.8 | 4.2 | 2.7 | 5.7 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
スパイク回収
The spike recovery was evaluated by spiking 3 levels of human CHIT1 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 83% to 115% with an overall mean recovery of 97%.
サンプル値
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 0.24-11.50 | 100 | 4.04 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of CHIT1 in this assay. No medical histories were available for the donors.
実験タイプ
種属反応性
検出方法
所要時間
標準曲線梯度
インキュベーション回数
2
Detectable Sample
サンプル添加量
製品の特徴
保存条件
2-8 ℃で保存
ターゲット
UNIPROT #
別名
CHI3
CHIT
CHIT1
CHIT1_HUMAN
CHITD
Chitinase 1
Chitinase 1 (chitotriosidase)
Chitinase-1
Chitotriosidase
Chitotriosidase-1
展開