Human Adiponectinenzyme-linked immunoassay kit（one step）
Catalog# EHY0099
Human Adiponectinenzyme-linked immunoassay kit（one step）
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ELISA
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Human
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15.63~1000 ng/ml
製品概要
製品名
Human Adiponectinenzyme-linked immunoassay kit（one step）
製品タイプ
Elisa Kit
検出感度
0.14 ng/ml (10 μl)
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0079 | 0.0072 | 0.0076 | |
| 15.63 | 0.0964 | 0.0988 | 0.0976 | 0.0901 |
| 31.25 | 0.1636 | 0.1991 | 0.1814 | 0.1738 |
| 62.50 | 0.3482 | 0.3617 | 0.3550 | 0.3474 |
| 125.00 | 0.7514 | 0.7524 | 0.7519 | 0.7444 |
| 250.00 | 1.5100 | 1.5200 | 1.5150 | 1.5075 |
| 500.00 | 2.0590 | 2.4110 | 2.2350 | 2.2275 |
| 1000.00 | 3.6840 | 3.3470 | 3.5155 | 3.5080 |
精度
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (ng/ml) | 13.5 | 75.9 | 218.3 | 16.5 | 79.5 | 230.5 |
| Standard Deviation | 0.9 | 5.8 | 13.3 | 1.2 | 5.7 | 9.6 |
| Coefficient of Variation (%) | 6.7 | 7.6 | 6.1 | 7.1 | 7.1 | 4.2 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
スパイク回収
The spike recovery was evaluated by spiking 3 levels of human Adiponectin into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 87% to 119% with an overall mean recovery of 103%.
サンプル値
| Sample Matrix | Sample Evaluated | Range (μg/ml) | Detectable (%) | Mean of Detectable (μg/ml) |
|---|---|---|---|---|
| Serum | 30 | 1.63-6.61 | 100 | 3.93 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of Adiponectin in this assay. No medical histories were available for the donors.
実験タイプ
種属反応性
検出方法
所要時間
標準曲線梯度
インキュベーション回数
2
Detectable Sample
サンプル添加量
製品の特徴
保存条件
2-8 ℃で保存
ターゲット
UNIPROT #
別名
30 kDa adipocyte complement related protein
30 kDa adipocyte complement-related protein
ACDC
Acrp 30
ACRP30
ADIPO_HUMAN
Adipocyte
Adipocyte C1q and collagen domain containing protein
Adipocyte complement related 30 kDa protein
Adipocyte complement related protein of 30 kDa
展開