Human Apolipoprotein E/Apo E enzyme-linked immunoassay kit
Catalog# EH0237
Human Apolipoprotein E/Apo E enzyme-linked immunoassay kit
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ELISA
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Human
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156.25~10000 pg/ml
製品概要
製品名
Human Apolipoprotein E/Apo E enzyme-linked immunoassay kit
製品タイプ
Elisa Kit
検出感度
125.76 pg/ml (50 μl)；156.06 pg/ml (10 μl)；
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0526 | 0.0539 | 0.0533 | |
| 156.25 | 0.1709 | 0.1699 | 0.1704 | 0.1172 |
| 312.50 | 0.3084 | 0.2913 | 0.2999 | 0.2466 |
| 625.00 | 0.5639 | 0.5584 | 0.5612 | 0.5079 |
| 1250.00 | 1.1400 | 1.1780 | 1.1590 | 1.1058 |
| 2500.00 | 2.2340 | 2.1280 | 2.1810 | 2.1278 |
| 5000.00 | 3.8460 | 3.8170 | 3.8315 | 3.7783 |
| 10000.00 | 4.5529 | 4.5239 | 4.5384 | 4.4852 |
精度
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 20.9 | 124.5 | 383.8 | 20.0 | 136.7 | 398.5 |
| Standard Deviation | 1.6 | 9.3 | 27.8 | 1.1 | 6.8 | 9.7 |
| Coefficient of Variation (%) | 7.6 | 7.5 | 7.3 | 5.5 | 5.0 | 2.4 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
スパイク回収
The spike recovery was evaluated by spiking 3 levels of human Apolipoprotein E/Apo E into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 119% with an overall mean recovery of 106%.
サンプル値
| Sample Matrix | Sample Evaluated | Range (μg/ml) | Detectable (%) | Mean of Detectable (μg/ml) |
|---|---|---|---|---|
| Serum | 30 | 23.36-55.33 | 100 | 37.04 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated in this assay. No medical histories were available for the donors.
実験タイプ
種属反応性
検出方法
所要時間
標準曲線梯度
インキュベーション回数
2
Detectable Sample
サンプル添加量
製品の特徴
保存条件
2-8 ℃で保存