Human LAMC2 enzyme-linked immunoassay kit

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Catalog# EH0220

Human LAMC2 enzyme-linked immunoassay kit

Application

ELISA

Reactivity

Human

Standard curve range

13.72~10000 pg/ml

製品概要

製品名

Human LAMC2 enzyme-linked immunoassay kit

製品タイプ

Elisa Kit

検出感度

0.17 pg/ml (50 μl)；2.50 pg/ml (10 μl)；

pg/ml	O.D.		Average	Corrected
0.00	0.0294	0.0294	0.0294
13.72	0.0333	0.0390	0.0362	0.0068
41.15	0.0455	0.0522	0.0489	0.0195
123.46	0.0964	0.0886	0.0925	0.0631
370.37	0.2276	0.2279	0.2278	0.1984
1111.11	0.6936	0.6883	0.6910	0.6616
3333.33	2.0760	2.0380	2.0570	2.0276
10000.00	3.9370	4.0532	3.9951	3.9657

精度

	Intra-Assay Precision			Inter-Assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average (pg/ml)	150.6	785.4	2461.7	150.3	798.8	2646.6
Standard Deviation	7.0	21.8	103.1	7.5	14.7	96.5
Coefficient of Variation (%)	4.7	2.8	4.2	5	1.8	3.6

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

スパイク回収

The spike recovery was evaluated by spiking 3 levels of human LAMC2 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 79% to 118% with an overall mean recovery of 94%.

サンプル値

Sample Matrix	Sample Evaluated	Range (pg/ml)	Detectable (%)	Mean of Detectable (pg/ml)
Serum	30	n.d.-47.67	18.8	21.95

Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated in this assay. No medical histories were available for the donors.

実験タイプ

Sandwich Elisa

種属反応性

Human

検出方法

Colorimetric

所要時間

120min

標準曲線梯度

7点/3倍

インキュベーション回数

Detectable Sample

可溶性物质的液相样品。例如：血清，血浆，细胞培养上清，组织研磨液等。

サンプル添加量

50 μl/10 μl

製品の特徴

保存条件

2-8 ℃で保存

ターゲット

UNIPROT #

SwissProt: Q13753 Human

機能

Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, presumably reflecting diverse functions in vivo. This gene encodes the gamma chain isoform laminin, gamma 2. The gamma 2 chain, formerly thought to be a truncated version of beta chain (B2t), is highly homologous to the gamma 1 chain; however, it lacks domain VI, and domains V, IV and III are shorter. It is expressed in several fetal tissues but differently from gamma 1, and is specifically localized to epithelial cells in skin, lung and kidney. The gamma 2 chain together with alpha 3 and beta 3 chains constitute laminin 5 (earlier known as kalinin), which is an integral part of the anchoring filaments that connect epithelial cells to the underlying basement membrane. The epithelium-specific expression of the gamma 2 chain implied its role as an epithelium attachment molecule, and mutations in this gene have been associated with junctional epidermolysis bullosa, a skin disease characterized by blisters due to disruption of the epidermal-dermal junction. Two transcript variants resulting from alternative splicing of the 3' terminal exon, and encoding different isoforms of gamma 2 chain, have been described. The two variants are differentially expressed in embryonic tissues, however, the biological significance of the two forms is not known. Transcript variants utilizing alternative polyA_signal have also been noted in literature.

Human LAMC2 enzyme-linked immunoassay kit

お問い合わせ

製品概要

製品名

製品タイプ

検出感度

精度

スパイク回収

サンプル値

実験タイプ

種属反応性

検出方法

所要時間

標準曲線梯度

インキュベーション回数

Detectable Sample

サンプル添加量

製品の特徴

保存条件

ターゲット

UNIPROT #

機能

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