Human DKK1 enzyme-linked immunoassay kit
Catalog# EH0233
Human DKK1 enzyme-linked immunoassay kit
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ELISA
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Human
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13.72~10000 pg/ml
製品概要
製品名
Human DKK1 enzyme-linked immunoassay kit
製品タイプ
Elisa Kit
検出感度
1.37 pg/ml (50 μl)；10.35 pg/ml (10 μl)；
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0096 | 0.0093 | 0.0095 | |
| 13.72 | 0.0204 | 0.0206 | 0.0205 | 0.0111 |
| 41.15 | 0.0493 | 0.0476 | 0.0485 | 0.0390 |
| 123.46 | 0.1434 | 0.1343 | 0.1389 | 0.1294 |
| 370.37 | 0.4189 | 0.4093 | 0.4141 | 0.4047 |
| 1111.11 | 1.1580 | 1.1070 | 1.1325 | 1.1231 |
| 3333.33 | 2.5370 | 2.4540 | 2.4955 | 2.4861 |
| 10000.00 | 3.7700 | 3.7340 | 3.7520 | 3.7426 |
精度
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 214.0 | 991.5 | 3116.4 | 191.8 | 950.2 | 3209.4 |
| Standard Deviation | 7.2 | 33.5 | 130.7 | 5.3 | 23.7 | 122.8 |
| Coefficient of Variation (%) | 3.3 | 3.4 | 4.2 | 2.8 | 2.5 | 3.8 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
スパイク回収
The spike recovery was evaluated by spiking 3 levels of human DKK1 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 105% to 120% with an overall mean recovery of 113%.
サンプル値
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 2716.70-5354.17 | 100 | 3894.92 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated in this assay. No medical histories were available for the donors.
実験タイプ
種属反応性
検出方法
所要時間
標準曲線梯度
インキュベーション回数
2
Detectable Sample
サンプル添加量
製品の特徴
保存条件
2-8 ℃で保存