Human MERTK enzyme-linked immunoassay kit（one step）
Catalog# EHY0189
Human MERTK enzyme-linked immunoassay kit（one step）
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ELISA
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Human
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0.27~200 ng/ml
製品概要
製品名
Human MERTK enzyme-linked immunoassay kit（one step）
製品タイプ
Elisa Kit
検出感度
0.03 ng/ml (50 μl)；0.41 ng/ml (10 μl)；
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0114 | 0.0112 | 0.0113 | |
| 0.27 | 0.0191 | 0.0184 | 0.0188 | 0.0075 |
| 0.82 | 0.0343 | 0.0338 | 0.0341 | 0.0228 |
| 2.47 | 0.0751 | 0.0732 | 0.0742 | 0.0629 |
| 7.41 | 0.1987 | 0.2044 | 0.2016 | 0.1903 |
| 22.22 | 0.4883 | 0.5378 | 0.5131 | 0.5018 |
| 66.67 | 1.4530 | 1.5690 | 1.5110 | 1.4997 |
| 200.00 | 3.4070 | 3.4100 | 3.4085 | 3.3972 |
精度
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (ng/ml) | 3.7 | 21.9 | 76.7 | 3.0 | 17.4 | 58.8 |
| Standard Deviation | 0.2 | 0.9 | 4.1 | 0.2 | 1.1 | 4.5 |
| Coefficient of Variation (%) | 5.5 | 4.0 | 5.3 | 5.6 | 6.1 | 7.7 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
スパイク回収
The spike recovery was evaluated by spiking 3 levels of human MERTK into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 70% to 105% with an overall mean recovery of 94%.
サンプル値
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 37.02-124.26 | 100 | 56.4 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of MERTK in this assay. No medical histories were available for the donors.
実験タイプ
種属反応性
検出方法
所要時間
標準曲線梯度
インキュベーション回数
2
Detectable Sample
サンプル添加量
製品の特徴
保存条件
2-8 ℃で保存
ターゲット
UNIPROT #
別名
c MER
c mer proto oncogene tyrosine kinase
c-mer
cMER
cmer protooncogene tyrosine kinase
Eyk
MER
MER receptor tyrosine kinase
MERK
MERPEN
展開