Human Granzyme A/GZMA enzyme-linked immunoassay kit
Catalog# EH0129
Human Granzyme A/GZMA enzyme-linked immunoassay kit
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ELISA
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Human
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2.74~2000 pg/ml
製品概要
製品名
Human Granzyme A/GZMA enzyme-linked immunoassay kit
製品タイプ
Elisa Kit
検出感度
0.46 pg/ml (50 μl)；1.46 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0136 | 0.0141 | 0.0139 | |
| 2.74 | 0.0255 | 0.0239 | 0.0247 | 0.0109 |
| 8.23 | 0.0392 | 0.0410 | 0.0401 | 0.0263 |
| 24.69 | 0.0845 | 0.0824 | 0.0835 | 0.0696 |
| 74.07 | 0.2141 | 0.2112 | 0.2127 | 0.1988 |
| 222.22 | 0.6253 | 0.5911 | 0.6082 | 0.5944 |
| 666.67 | 1.7910 | 1.7440 | 1.7675 | 1.7537 |
| 2000.00 | 3.7830 | 3.7900 | 3.7865 | 3.7727 |
精度
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 49.6 | 198.7 | 609.8 | 47.5 | 189.0 | 608.9 |
| Standard Deviation | 2.3 | 8.7 | 26.3 | 1.5 | 7.3 | 20.0 |
| Coefficient of Variation (%) | 4.6 | 4.4 | 4.3 | 3.2 | 3.9 | 3.3 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
スパイク回収
The spike recovery was evaluated by spiking 3 levels of human Granzyme A into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 81% to 97% with an overall mean recovery of 91%.
サンプル値
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 62.47-179.55 | 100.0 | 113.58 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of Granzyme A in this assay. No medical histories were available for the donors.
実験タイプ
種属反応性
検出方法
所要時間
標準曲線梯度
インキュベーション回数
2
Detectable Sample
サンプル添加量
製品の特徴
保存条件
2-8 ℃で保存
ターゲット
UNIPROT #
別名
CTL tryptase
CTLA3
Cytolytic t cell and natural killer cell specific trypsin like serine protease
Cytotoxic T lymphocyte associated serine esterase 3
Cytotoxic T lymphocyte proteinase 1
Cytotoxic T-lymphocyte proteinase 1
Fragmentin 1
Fragmentin-1
GRAA_HUMAN
Granzyme 1 cytotoxic T lymphocyte associated serine esterase 3
展開