Human HGF enzyme-linked immunoassay kit
Catalog# EH0019
Human HGF enzyme-linked immunoassay kit
-
ELISA
-
Human
-
10.97~8000 pg/ml
-
unconjugated
製品概要
製品名
Human HGF enzyme-linked immunoassay kit
製品タイプ
Elisa Kit
検出感度
4.07 pg/ml (50 μl)；6.01 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0284 | 0.0277 | 0.0281 | |
| 10.97 | 0.0360 | 0.0382 | 0.0371 | 0.0091 |
| 32.92 | 0.0521 | 0.0501 | 0.0511 | 0.0231 |
| 98.77 | 0.1109 | 0.0958 | 0.1034 | 0.0753 |
| 296.30 | 0.2883 | 0.2774 | 0.2829 | 0.2548 |
| 888.89 | 0.8003 | 0.8033 | 0.8018 | 0.7738 |
| 2666.67 | 2.3398 | 2.3380 | 2.3389 | 2.3109 |
| 8000.00 | 3.9661 | 4.0435 | 4.0048 | 3.9768 |
精度
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 190.1 | 1075.3 | 3244.1 | 214.2 | 1168.4 | 3780.4 |
| Standard Deviation | 12.4 | 73.9 | 219.8 | 15.2 | 69.0 | 144.5 |
| Coefficient of Variation (%) | 6.5 | 6.9 | 6.8 | 7.1 | 5.9 | 3.8 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
スパイク回収
The spike recovery was evaluated by spiking 3 levels of human HGF into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 88% to 121% with an overall mean recovery of 108%.
サンプル値
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 360.14-1072.52 | 100 | 609.53 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of HGF in this assay. No medical histories were available for the donors.
実験タイプ
種属反応性
検出方法
所要時間
標準曲線梯度
インキュベーション回数
2
Detectable Sample
サンプル添加量
製品の特徴
保存条件
2-8 ℃で保存
ターゲット
UNIPROT #
別名
DFNB39
F TCF
Fibroblast derived tumor cytotoxic factor
Hepatocyte growth factor (hepapoietin A
scatter factor)
Hepatocyte growth factor
Hepatocyte growth factor beta chain
Hepatocyte growth factor precursor
Hepatopoietin A
Hepatopoietin-A
展開