Human CD47 enzyme-linked immunoassay kit
Catalog# EH0136
Human CD47 enzyme-linked immunoassay kit
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ELISA
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Human
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6.86~5000 pg/ml
製品概要
製品名
Human CD47 enzyme-linked immunoassay kit
製品タイプ
Elisa Kit
検出感度
1.44 pg/ml (50 μl)；3.63 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0092 | 0.0099 | 0.0096 | |
| 6.86 | 0.0223 | 0.0214 | 0.0219 | 0.0123 |
| 20.58 | 0.0512 | 0.0474 | 0.0493 | 0.0398 |
| 61.73 | 0.1392 | 0.1363 | 0.1378 | 0.1282 |
| 185.19 | 0.4027 | 0.3906 | 0.3967 | 0.3871 |
| 555.56 | 1.1000 | 1.0768 | 1.0884 | 1.0789 |
| 1666.67 | 2.9040 | 2.7840 | 2.8440 | 2.8345 |
| 5000.00 | 4.4723 | 4.4856 | 4.4790 | 4.4694 |
精度
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 106.6 | 559.2 | 2075.7 | 108.7 | 524.7 | 1653.5 |
| Standard Deviation | 3.1 | 16.8 | 73.7 | 4.0 | 20.3 | 69.5 |
| Coefficient of Variation (%) | 2.9 | 3.0 | 3.5 | 3.7 | 3.9 | 4.2 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
スパイク回収
The spike recovery was evaluated by spiking 3 levels of human CD47 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 95% to 119% with an overall mean recovery of 112%.
サンプル値
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 2.42-31.11 | 100 | 9.34 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of CD47 in this assay. No medical histories were available for the donors.
n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
実験タイプ
種属反応性
検出方法
所要時間
標準曲線梯度
インキュベーション回数
2
Detectable Sample
サンプル添加量
製品の特徴
保存条件
2-8 ℃で保存
ターゲット
UNIPROT #
別名
Antigen identified by monoclonal 1D8
Antigenic surface determinant protein OA3
CD 47
Cd47
CD47 antigen (Rh-related antigen, integrin-associated signal transducer)
CD47 antigen
CD47 glycoprotein
CD47 molecule
CD47_HUMAN
IAP
展開