Human CD137/4-1BB/TNFRSF9 enzyme-linked immunoassay kit

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Catalog# EH0124

Human CD137/4-1BB/TNFRSF9 enzyme-linked immunoassay kit

Application

ELISA

Reactivity

Human

Standard curve range

15.63~1000 pg/ml

製品概要

製品名

Human CD137/4-1BB/TNFRSF9 enzyme-linked immunoassay kit

製品タイプ

Elisa Kit

検出感度

0.47 pg/ml (50 μl)；5.07 pg/ml (10 μl)

pg/ml	O.D.		Average	Corrected
0.00	0.0659	0.0698	0.0679
15.63	0.1978	0.2148	0.2063	0.1385
31.25	0.3346	0.3512	0.3429	0.2751
62.50	0.5916	0.6533	0.6225	0.5546
125.00	1.1130	1.1470	1.1300	1.0622
250.00	1.8960	1.9670	1.9315	1.8637
500.00	3.2850	3.4530	3.3690	3.3012
1000.00	4.3356	4.3766	4.3561	4.2883

精度

	Intra-Assay Precision			Inter-Assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average (pg/ml)	25.6	117.0	352.9	22.8	110.2	319.1
Standard Deviation	1.4	3.5	10.2	1.1	2.3	12.4
Coefficient of Variation (%)	5.3	3.0	2.9	4.7	2.1	3.9

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

スパイク回収

The spike recovery was evaluated by spiking 3 levels of human CD137/4-1BB/TNFRSF9 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 86% to 116% with an overall mean recovery of 103%.

サンプル値

Sample Matrix	Sample Evaluated	Range (pg/ml)	Detectable (%)	Mean of Detectable (pg/ml)
Serum	30	20.14-49.28	100	32.58

Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of CD137/4-1BB/TNFRSF9 in this assay. No medical histories were available for the donors.

実験タイプ

Sandwich Elisa

種属反応性

Human

検出方法

Colorimetric

所要時間

120min

標準曲線梯度

7点

インキュベーション回数

Detectable Sample

可溶性物质的液相样品。例如：血清，血浆，细胞培养上清，组织研磨液等。

サンプル添加量

50 μl/10 μl

製品の特徴

保存条件

2-8 ℃で保存

ターゲット

UNIPROT #

SwissProt: Q07011 Human

別名

4 1BB

4 1BB ligand receptor

4-1BB ligand receptor

4-1BB Ligand Receptor T Cell

4-1BB, mouse, homolog of

Antigen 4-1BB Homolog

CD 137

CD137

CD137 antigen

CDw137

展開

機能

4-1BB is an inducible T cell surface protein belonging to the TNF receptor superfamily. It is alternatively known as TNFRSF9, CD137, and ILA. The 255 amino acid human 4-1BB is a type I transmembrane protein having in its extracellular domain four of the cysteine-rich motifs that are characteristic of the TNF receptor superfamily. The 30 kDa glycoprotein exists both as a monomer and as a dimer on T cells. The human and mouse proteins share 60% amino acid identity. 4-1BB is absent from naive T cells, but it is upregulated and continually expressed following T cell activation. The natural ligand, 4-1BBL, is a member of the TNF superfamily and is expressed on activated antigen presenting cells including dendritic cells, macrophages, and B cells. Cross-linking of 4-1BB by 4-1BBL or by agonistic antibodies transmits a potent co-stimulatory signal that enhances the effect of other activating signals such as PHA or anti-CD3 antibodies. 4-1BB signals through the TFAF2‑NIK pathway resulting in activation of NF-kappa B and ultimately promoting proliferation and survival of T cells.

Human CD137/4-1BB/TNFRSF9 enzyme-linked immunoassay kit

お問い合わせ

製品概要

製品名

製品タイプ

検出感度

精度

スパイク回収

サンプル値

実験タイプ

種属反応性

検出方法

所要時間

標準曲線梯度

インキュベーション回数

Detectable Sample

サンプル添加量

製品の特徴

保存条件

ターゲット

UNIPROT #

別名

機能

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