Human CXCL1/GRO alpha enzyme-linked immunoassay kit
Catalog# EH0054
Human CXCL1/GRO alpha enzyme-linked immunoassay kit
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ELISA
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Human
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20.48~5000 pg/ml
-
unconjugated
製品概要
製品名
Human CXCL1/GRO alpha enzyme-linked immunoassay kit
製品タイプ
Elisa Kit
検出感度
14.89 pg/ml (50 μl)；32.67 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0137 | 0.0129 | 0.0133 | |
| 20.48 | 0.0170 | 0.0161 | 0.0166 | 0.0033 |
| 51.20 | 0.0261 | 0.0234 | 0.0248 | 0.0115 |
| 128.00 | 0.0446 | 0.0443 | 0.0445 | 0.0312 |
| 320.00 | 0.1492 | 0.1474 | 0.1483 | 0.1350 |
| 800.00 | 0.4857 | 0.5021 | 0.4939 | 0.4806 |
| 2000.00 | 1.2570 | 1.2630 | 1.2600 | 1.2467 |
| 5000.00 | 2.2760 | 2.2510 | 2.2635 | 2.2502 |
精度
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 119.3 | 509.0 | 1409.9 | 110.0 | 510.1 | 1498.1 |
| Standard Deviation | 9.4 | 39.3 | 109.4 | 5.4 | 27.0 | 100.4 |
| Coefficient of Variation (%) | 7.9 | 7.7 | 7.8 | 4.9 | 5.3 | 6.7 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
スパイク回収
The spike recovery was evaluated by spiking 3 levels of human CXCL1 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 72% to 125% with an overall mean recovery of 101%.
サンプル値
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 77.28-616.13 | 100 | 358.62 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of CXCL1 in this assay. No medical histories were available for the donors.
実験タイプ
種属反応性
検出方法
所要時間
標準曲線梯度
インキュベーション回数
2
Detectable Sample
サンプル添加量
製品の特徴
保存条件
2-8 ℃で保存
ターゲット
UNIPROT #
別名
C-X-C motif chemokine 1
Chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity
alpha)
chemokine (C-X-C motif) ligand 1
CINC-1
CXCL1
Cytokine-induced neutrophil chemoattractant 1
Fibroblast secretory protein
Fsp
Gro 1
展開