Human RBP4 enzyme-linked immunoassay kit（one step）
Catalog# EHY0212
Human RBP4 enzyme-linked immunoassay kit（one step）
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ELISA
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Human
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0.14~100 ng/ml
製品概要
製品名
Human RBP4 enzyme-linked immunoassay kit（one step）
製品タイプ
Elisa Kit
検出感度
0.002 ng/ml (50 μl)；0.03 ng/ml (10 μl)；
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0087 | 0.0084 | 0.0086 | |
| 0.14 | 0.0340 | 0.0339 | 0.0340 | 0.0254 |
| 0.41 | 0.0873 | 0.0877 | 0.0875 | 0.0790 |
| 1.23 | 0.2276 | 0.2253 | 0.2265 | 0.2179 |
| 3.70 | 0.6074 | 0.5874 | 0.5974 | 0.5889 |
| 11.11 | 1.4550 | 1.3330 | 1.3940 | 1.3855 |
| 33.33 | 3.1820 | 3.2770 | 3.2295 | 3.2210 |
| 100.00 | 4.0330 | 4.2409 | 4.1370 | 4.1284 |
精度
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (ng/ml) | 1.2 | 9.1 | 33.5 | 1.3 | 12.6 | 34.1 |
| Standard Deviation | 0.1 | 0.5 | 2.5 | 0.1 | 0.4 | 1.4 |
| Coefficient of Variation (%) | 4.9 | 5.8 | 5.4 | 5.9 | 3.1 | 4.0 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
スパイク回収
The spike recovery was evaluated by spiking 3 levels of human RBP4 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 88% to 118% with an overall mean recovery of 100%.
サンプル値
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 278.09-774.24 | 100 | 534.25 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of RBP4 in this assay. No medical histories were available for the donors.
実験タイプ
種属反応性
検出方法
所要時間
標準曲線梯度
インキュベーション回数
2
Detectable Sample
サンプル添加量
製品の特徴
保存条件
2-8 ℃で保存
ターゲット
UNIPROT #
別名
OTTHUMP00000020114
OTTHUMP00000020115
OTTHUMP00000020116
Plasma retinol binding protein 4
Plasma retinol-binding protein
Plasma retinol-binding protein(1-176)
PRBP
PRO2222
RBP
RBP4
展開