Human tPA enzyme-linked immunoassay kit(one step)
Catalog# EHY0158
Human tPA enzyme-linked immunoassay kit(one step)
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ELISA
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Human
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0.14~100 ng/ml
製品概要
製品名
Human tPA enzyme-linked immunoassay kit(one step)
製品タイプ
Elisa Kit
検出感度
0.03 ng/ml (50 μl)；0.10 ng/ml (10 μl)；
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0058 | 0.0064 | 0.0061 | |
| 0.14 | 0.0107 | 0.0096 | 0.0102 | 0.0041 |
| 0.41 | 0.0195 | 0.0181 | 0.0188 | 0.0127 |
| 1.23 | 0.0457 | 0.0450 | 0.0454 | 0.0393 |
| 3.70 | 0.1406 | 0.1261 | 0.1334 | 0.1273 |
| 11.11 | 0.4191 | 0.3776 | 0.3984 | 0.3923 |
| 33.33 | 1.1900 | 1.1700 | 1.1800 | 1.1739 |
| 100.00 | 2.8370 | 2.8780 | 2.8575 | 2.8514 |
精度
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (ng/ml) | 1.8 | 9.0 | 29.2 | 1.8 | 9.4 | 31.7 |
| Standard Deviation | 0.1 | 0.4 | 1.5 | 0.1 | 0.4 | 1.8 |
| Coefficient of Variation (%) | 5.7 | 4.6 | 5.1 | 5.2 | 3.9 | 5.6 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
スパイク回収
The spike recovery was evaluated by spiking 3 levels of human tPA into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 97% to 115% with an overall mean recovery of 104%.
サンプル値
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 3.24-20.65 | 100 | 9.25 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of tPA in this assay. No medical histories were available for the donors.
実験タイプ
種属反応性
検出方法
所要時間
標準曲線梯度
インキュベーション回数
2
Detectable Sample
サンプル添加量
製品の特徴
保存条件
2-8 ℃で保存
ターゲット
UNIPROT #
別名
Alteplase
DKFZp686I03148
Plasminogen activator tissue
Plasminogen activator tissue type
PLAT
Reteplase
t PA
T Plasminogen Activator
t-PA
T-plasminogen activator
展開