Human CD85d enzyme-linked immunoassay kit
Catalog# EH0046
Human CD85d enzyme-linked immunoassay kit
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ELISA
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Human
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312.5~20000 pg/ml
製品概要
製品名
Human CD85d enzyme-linked immunoassay kit
製品タイプ
Elisa Kit
検出感度
10.40 pg/ml (50 μl)：14.80 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0345 | 0.0329 | 0.0337 | |
| 312.50 | 0.0936 | 0.0922 | 0.0929 | 0.0592 |
| 625.00 | 0.1418 | 0.1472 | 0.1445 | 0.1108 |
| 1250.00 | 0.2482 | 0.2518 | 0.2500 | 0.2163 |
| 2500.00 | 0.4908 | 0.4994 | 0.4951 | 0.4614 |
| 5000.00 | 0.9079 | 0.9281 | 0.9180 | 0.8843 |
| 10000.00 | 1.8010 | 1.8280 | 1.8145 | 1.7808 |
| 20000.00 | 3.2730 | 3.2200 | 3.2465 | 3.2128 |
精度
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 278.4 | 1676.4 | 5340.2 | 335.2 | 1676.4 | 5347.2 |
| Standard Deviation | 18.3 | 50.3 | 193.0 | 17.5 | 80.8 | 160.4 |
| Coefficient of Variation (%) | 6.6 | 3.0 | 3.6 | 5.2 | 4.8 | 3.0 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
スパイク回収
The spike recovery was evaluated by spiking 3 levels of human CD85d into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 127% with an overall mean recovery of 112%.
サンプル値
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 2.46-9.25 | 100 | 5.35 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of IL-12/IL-23 p40 in this assay. No medical histories were available for the donors.
実験タイプ
種属反応性
検出方法
所要時間
標準曲線梯度
インキュベーション回数
2
Detectable Sample
サンプル添加量
製品の特徴
保存条件
2-8 ℃で保存
ターゲット
UNIPROT #
別名
CD85 antigen-like family member D
CD85d
CD85d antigen
ILT 4
ILT-4
ILT4
Immunoglobulin like transcript 4
Immunoglobulin-like transcript 4
Leukocyte immunoglobulin like receptor 2
Leukocyte immunoglobulin like receptor subfamily B member 2
展開