Human IGFBP1 enzyme-linked immunoassay kit(one step)

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Catalog# EHY0258

Human IGFBP1 enzyme-linked immunoassay kit(one step)

Application

ELISA

Reactivity

Human

Standard curve range

13.72~10000 pg/ml

製品概要

製品名

Human IGFBP1 enzyme-linked immunoassay kit(one step)

製品タイプ

Elisa Kit

検出感度

1.13 pg/ml (50 μl)；15.75 pg/ml (10 μl)；

pg/ml	O.D.		Average	Corrected
0.00	0.0057	0.0053	0.0055
13.72	0.0083	0.0082	0.0083	0.0028
41.15	0.0165	0.0154	0.0160	0.0105
123.46	0.0426	0.0463	0.0445	0.0390
370.37	0.1283	0.1181	0.1232	0.1177
1111.11	0.3838	0.3516	0.3677	0.3622
3333.33	1.0460	0.9522	0.9991	0.9936
10000.00	2.3570	2.2190	2.2880	2.2825

精度

	Intra-Assay Precision			Inter-Assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average (pg/ml)	236.9	1109.1	3258.2	230.4	1101.7	3259.2
Standard Deviation	16.1	73.4	254.8	4.6	64.8	84.5
Coefficient of Variation (%)	6.8	6.5	7.8	2.8	3.0	3.0

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

スパイク回収

The spike recovery was evaluated by spiking 3 levels of Human IGFBP1 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 90% to 119% with an overall mean recovery of 105%.

サンプル値

Sample Matrix	Sample Evaluated	Range (ng/ml)	Detectable (%)	Mean of Detectable (ng/ml)
Serum	30	0.72-26.08	100	8.56

Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of Human IGFBP1 in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.

実験タイプ

Sandwich Elisa

種属反応性

Human

検出方法

Colorimetric

所要時間

60min

標準曲線梯度

7点/3倍

インキュベーション回数

Detectable Sample

血清，血浆

サンプル添加量

50 μl/10 μl

製品の特徴

保存条件

2-8 ℃で保存

ターゲット

UNIPROT #

SwissProt: P08833 Human

機能

The superfamily of insulin-like growth factor (IGF) binding proteins include the six high-affinity IGF binding proteins (IGFBP) and at least four additional low-affinity binding proteins referred to as IGFBP related proteins (IGFBP-rP). All IGFBP superfamily members are cysteine-rich proteins with conserved cysteine residues, which are clustered in the amino- and carboxy-terminal thirds of the molecule. IGFBPs modulate the biological activities of IGF proteins. Some IGFBPs may also have intrinsic bioactivity that is independent of their ability to bind IGF proteins. Post-translational modifications of IGFBP, including glycosylation, phosphorylation and proteolysis, have been shown to modify the affinities of the binding proteins to IGF. ALS (Acid Labile Subunit) is a liver-derived protein that exists in a ternary complex with Insulin-like Growth Factor (IGF)-binding Protein-3 (IGFBP-3) or IGFBP-5, and either IGF-I or IGF-II. ALS increases the half-life of IGF/IGFBP complexes in circulation.

Human IGFBP1 enzyme-linked immunoassay kit(one step)

お問い合わせ

製品概要

製品名

製品タイプ

検出感度

精度

スパイク回収

サンプル値

実験タイプ

種属反応性

検出方法

所要時間

標準曲線梯度

インキュベーション回数

Detectable Sample

サンプル添加量

製品の特徴

保存条件

ターゲット

UNIPROT #

機能

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