Human CD115/M-CSF R/CSF-1-R enzyme-linked immunoassay kit（one step）

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Catalog# EHY0168

Human CD115/M-CSF R/CSF-1-R enzyme-linked immunoassay kit（one step）

Application

ELISA

Reactivity

Human

Standard curve range

0.41~300 ng/ml

製品概要

製品名

Human CD115/M-CSF R/CSF-1-R enzyme-linked immunoassay kit（one step）

製品タイプ

Elisa Kit

検出感度

0.01 ng/ml (50 μl)；0.08 ng/ml (10 μl)；

ng/ml	O.D.		Average	Corrected
0.00	0.0056	0.0054	0.0055
0.41	0.0188	0.0196	0.0192	0.0137
1.23	0.0493	0.0501	0.0497	0.0442
3.70	0.1445	0.1453	0.1449	0.1394
11.11	0.4360	0.4285	0.4323	0.4268
33.33	1.2330	1.2430	1.2380	1.2325
100.00	3.2110	3.1820	3.1965	3.1910
300.00	4.2906	4.2706	4.2806	4.2751

精度

	Intra-Assay Precision			Inter-Assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average (ng/ml)	7.6	38.8	148.5	6.5	33.6	102.7
Standard Deviation	0.3	1.8	2.7	0.4	2.0	2.5
Coefficient of Variation (%)	3.6	4.5	1.8	6.0	6.0	2.5

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

スパイク回収

The spike recovery was evaluated by spiking 3 levels of human CD115/M-CSF R/CSF-1-R into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 99% with an overall mean recovery of 89%.

サンプル値

Sample Matrix	Sample Evaluated	Range (ng/ml)	Detectable (%)	Mean of Detectable (ng/ml)
Serum	30	174.98-741.02	100	502.94

Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of CD115/M-CSF R/CSF-1-R in this assay. No medical histories were available for the donors.

実験タイプ

Sandwich Elisa

種属反応性

Human

検出方法

Colorimetric

所要時間

60min

標準曲線梯度

7点/3倍

インキュベーション回数

Detectable Sample

血清，血浆

サンプル添加量

50 μl/10 μl

製品の特徴

保存条件

2-8 ℃で保存

ターゲット

UNIPROT #

SwissProt: P07333 Human

別名

C FMS

CD 115

CD115

CD115 antigen

CFMS

Colony stimulating factor 1 receptor

Colony stimulating factor I receptor

CSF 1 R

CSF 1R

CSF-1 receptor

展開

機能

M-CSF receptor, the product of the c-fms proto-oncogene, is a member of the type III subfamily of receptor tyrosine kinases that also includes receptors for SCF and PDGF. These receptors each contain five immunoglobulin-like domains in their extracellular domain (ECD) and a split kinase domain in their intracellular region. M-CSF receptor is expressed primarily on cells of the monocyte/macrophage lineage, dendritic cells, stem cells and in the developing placenta. Human M-CSF receptor cDNA encodes a 972 amino acid (aa) type I membrane protein with a 19 aa signal peptide, a 493 aa extracellular region containing the ligand-binding domain, a 25 aa transmembrane domain and a 435 aa cytoplasmic domain. The human M-CSF R ECD shares 60%, 64%, 72%, 75%, 75% and 76% aa identity with mouse, rat, bovine, canine, feline and equine M-CSF R, respectively. Activators of protein kinase C induce TACE/ADAM17 cleavage of the M-CSF receptor, releasing the functional ligand-binding extracellular domain. M-CSF binding induces receptor homodimerization, resulting in transphosphorylation of specific cytoplasmic tyrosine residues and signal transduction. The intracellular domain of activated M-CSF R binds more than 150 proteins that affect cell proliferation, survival, differentiation and cytoskeletal reorganization. Among these, PI3Kinase, P42/44 ERK and c-Cbl are key transducers of M-CSF R signals. M-CSF R engagement is continuously required for macrophage survival and regulates lineage decisions and maturation of monocytes, macrophages, osteoclasts and DC. M-CSF R and integrin alpha v beta 3 share signaling pathways during osteoclastogenesis and deletion of either causes osteopetrosis. In the brain, microglia expressing increased M-CSF R are concentrated with Alzheimers a beta peptide, but their role in pathogenesis is unclear.

Human CD115/M-CSF R/CSF-1-R enzyme-linked immunoassay kit（one step）

お問い合わせ

製品概要

製品名

製品タイプ

検出感度

精度

スパイク回収

サンプル値

実験タイプ

種属反応性

検出方法

所要時間

標準曲線梯度

インキュベーション回数

Detectable Sample

サンプル添加量

製品の特徴

保存条件

ターゲット

UNIPROT #

別名

機能

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