Human Galectin3 enzyme-linked immunoassay kit

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Catalog# EH0286

Human Galectin3 enzyme-linked immunoassay kit

Application

ELISA

Reactivity

Human

Standard curve range

312.50~20000 pg/ml

製品概要

製品名

Human Galectin3 enzyme-linked immunoassay kit

製品タイプ

Elisa Kit

検出感度

125.02 pg/ml (50 μl)；404.56 pg/ml (10 μl)；

pg/ml	O.D.		Average	Corrected
0.00	0.0186	0.0177	0.0182
312.50	0.0266	0.0256	0.0261	0.0080
625.00	0.0389	0.0386	0.0388	0.0206
1250.00	0.0849	0.0847	0.0848	0.0667
2500.00	0.2597	0.2528	0.2563	0.2381
5000.00	0.7995	0.7769	0.7882	0.7701
10000.00	2.1670	2.1290	2.1480	2.1299
20000.00	3.8230	3.8410	3.8320	3.8139

精度

	Intra-Assay Precision			Inter-Assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average (pg/ml)	341.7	1933.2	5656.0	396.7	1924.8	5724.1
Standard Deviation	25.4	50.6	153.1	30.8	36.8	93.0
Coefficient of Variation (%)	7.4	2.6	2.7	7.8	1.9	1.6

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

スパイク回収

The spike recovery was evaluated by spiking 3 levels of human Galectin3 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 90% to 112% with an overall mean recovery of 108%.

サンプル値

Sample Matrix	Sample Evaluated	Range (ng/ml)	Detectable (%)	Mean of Detectable (ng/ml)
Serum	30	60.49-135.27	100.0	93.91

Serum/Plasma– Thirty samples from apparently healthy volunteers were evaluated for the presence of human Galectin3 in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.

実験タイプ

Sandwich Elisa

種属反応性

Human

検出方法

Colorimetric

所要時間

120min

標準曲線梯度

7点

インキュベーション回数

Detectable Sample

可溶性物质的液相样品。例如：血清，血浆，细胞培养上清，组织研磨液等。

サンプル添加量

50 μl/10 μl

製品の特徴

保存条件

2-8 ℃で保存

ターゲット

UNIPROT #

SwissProt: P17931 Human

機能

The galectins constitute a large family of carbohydrate-binding proteins with specificity for N-acetyl-lactosamine-containing glycoproteins. At least 14 mammalian galectins, which share structural similarities in their carbohydrate recognition domains (CRD), have been identified. The galectins have been classified into the prototype galectins (-1, -2, -5, -7, -10, -11, -13, -14), which contain one CRD and exist either as a monomer or a noncovalent homodimer; the chimera galectins (Galectin-3) containing one CRD linked to a nonlectin domain; and the tandem-repeat galectins (-4, -6, -8, -9, -12) consisting of two CRDs joined by a linker peptide. Galectins lack a classical signal peptide and can be localized to the cytosolic compartments where they have intracellular functions. However, via one or more as yet unidentified non-classical secretory pathways, galectins can also be secreted to function extracellularly. Individual members of the galectin family have different tissue distribution profiles and exhibit subtle differences in their carbohydrate-binding specificities. Each family member may preferentially bind to a unique subset of cell-surface glycoproteins. Galectin-3, also known as Mac-2, L29, CBP35, and epsilon BP, is a chimera galectin that has a tendency to dimerize. Besides the soluble protein, alternatively spliced forms of chicken Galectin-3 containing a transmembrane-spanning domain and a leucine zipper motif have been reported. Galectin-3 is expressed in tumor cells, macrophages, activated T cells, osteoclasts, epithelial cells, and fibroblasts. It binds various matrix glycoproteins including laminin, fibronectin, LAMPS, 90K/Mac-2BP, MP20, and CEA. Galectin-3 promotes cell growth and proliferation for many cell types. Galectin-3 acts intracellularly to prevent apoptosis. Depending on the cell types, Galectin-3 exhibits pro- or anti-adhesive properties. Galectin-3 has proinflammatory activities in vitro and in vivo. It induces pro-inflammatory and inhibits Th2 type cytokine production. Galectin-3 chemoattracts monocytes and macrophages. It activates and degranulates basophils and mast cells. Elevated circulating levels of Galectin-3 has been show to correlate with the malignant potential of several types of cancer, suggesting that Galectin-3 is also involved in tumor growth and metastasis. Human and mouse Galectin-3 shares approximately 80% amino acid sequence similarity.

Human Galectin3 enzyme-linked immunoassay kit

お問い合わせ

製品概要

製品名

製品タイプ

検出感度

精度

スパイク回収

サンプル値

実験タイプ

種属反応性

検出方法

所要時間

標準曲線梯度

インキュベーション回数

Detectable Sample

サンプル添加量

製品の特徴

保存条件

ターゲット

UNIPROT #

機能

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