Human beta-NGF enzyme-linked immunoassay kit

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Catalog# EH0065

Human beta-NGF enzyme-linked immunoassay kit

Application

ELISA

Reactivity

Human

Standard curve range

6.86~5000 pg/ml

Conjugation

unconjugated

製品概要

製品名

Human beta-NGF enzyme-linked immunoassay kit

製品タイプ

Elisa Kit

検出感度

1.91 pg/ml (50 μl)；3.27 pg/ml (10 μl)

pg/ml	O.D.		Average	Corrected
0.00	0.0172	0.0164	0.0168
6.86	0.0228	0.0234	0.0231	0.0063
20.58	0.0418	0.0405	0.0412	0.0244
61.73	0.1004	0.1016	0.1010	0.0842
185.19	0.2676	0.2957	0.2817	0.2649
555.56	0.7539	0.8159	0.7849	0.7681
1666.67	1.9450	1.9640	1.9545	1.9377
5000.00	3.3400	3.7610	3.5505	3.5337

精度

	Intra-Assay Precision			Inter-Assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average (pg/ml)	188.0	1692.3	2668.5	204.0	977.6	2851.8
Standard Deviation	11.4	74.7	142.2	11.8	43.6	106.6
Coefficient of Variation (%)	6.0	4.4	5.3	5.8	4.5	3.7

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

スパイク回収

The spike recovery was evaluated by spiking 3 levels of human beta-NGF into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 104% to 132% with an overall mean recovery of 117%.

サンプル値

Sample Matrix	Sample Evaluated	Range (pg/ml)	Detectable (%)	Mean of Detectable (pg/ml)
Serum	30	n.d.-17.92	87.5	7.23

Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of beta-NGF in this assay. No medical histories were available for the donors.

実験タイプ

Sandwich Elisa

種属反応性

Human

検出方法

Colorimetric

所要時間

120min

標準曲線梯度

7点

インキュベーション回数

Detectable Sample

可溶性物质的液相样品。例如：血清，血浆，细胞培养上清，组织研磨液等。

サンプル添加量

50 μl/10 μl

製品の特徴

保存条件

2-8 ℃で保存

ターゲット

UNIPROT #

SwissProt: P01138 Human

別名

Beta nerve growth factor

Beta NGF

Beta-nerve growth factor

Beta-NGF

HSAN5

MGC161426

MGC161428

Nerve growth factor (beta polypeptide)

Nerve growth factor

Nerve growth factor beta

展開

機能

NGF was initially isolated in the mouse submandibular gland as a 7S complex composed of three non-covalently linked subunits, alpha, beta, and gamma. Both the alpha and gamma subunits of NGF are members of the kallikrein family of serine proteases while the beta subunit, called beta -NGF or 2.5S NGF, exhibits all the biological activities ascribed to NGF. Recombinant human beta -NGF is a homodimer of two 120 amino acid polypeptides. The human protein shares approximately 90% homology at the amino acid level with both the mouse and rat beta -NGF and exhibits cross-species activity. NGF is a well-characterized neurotropic protein that plays a critical role in the development of sympathetic and some sensory neurons in the peripheral nervous system. In addition, NGF can also act in the central nervous system as a trophic factor for basal forebrain cholinergic neurons. NGF has also been shown to have biological effects on non-neuronal tissues. NGF is mitogenic for a factor‑dependent human erythroleukemic cell line, TF-1. NGF has been found to increase the number of mast cells in neonatal rats and to induce histamine release from peritoneal mast cells. NGF will enhance histamine release and strongly modulate the formation of lipid mediators by basophils in response to various stimuli. NGF will also induce the growth and differentiation of human B lymphocytes as well as suppress apoptosis of murine peritoneal neutrophils. These results, taken together, suggest that NGF is a pleiotropic cytokine which, in addition to its neurotropic activities, may have an important role in the regulation of the immune system.

Human beta-NGF enzyme-linked immunoassay kit

お問い合わせ

製品概要

製品名

製品タイプ

検出感度

精度

スパイク回収

サンプル値

実験タイプ

種属反応性

検出方法

所要時間

標準曲線梯度

インキュベーション回数

Detectable Sample

サンプル添加量

製品の特徴

保存条件

ターゲット

UNIPROT #

別名

機能

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