Human HVEM/TNFRSF14 enzyme-linked immunoassay kit

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Catalog# EH0107

Human HVEM/TNFRSF14 enzyme-linked immunoassay kit

Application

ELISA

Reactivity

Human

Standard curve range

13.72~10000 pg/ml

製品概要

製品名

Human HVEM/TNFRSF14 enzyme-linked immunoassay kit

製品タイプ

Elisa Kit

検出感度

1.68 pg/ml (50 μl)；1.86 pg/ml (10 μl)

pg/ml	O.D.		Average	Corrected
0.00	0.0193	0.0194	0.0194
13.72	0.0363	0.0381	0.0372	0.0179
41.15	0.0732	0.0746	0.0739	0.0546
123.46	0.1891	0.1851	0.1871	0.1678
370.37	0.5121	0.5123	0.5122	0.4929
1111.11	1.3600	1.3770	1.3685	1.3492
3333.33	2.8210	2.8440	2.8325	2.8132
10000.00	4.1600	4.0765	4.1183	4.0989

精度

	Intra-Assay Precision			Inter-Assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average (pg/ml)	222.9	1101.3	3269.9	214.7	1006.4	3038.2
Standard Deviation	11.7	44.4	201.4	8.3	50.4	164.1
Coefficient of Variation (%)	5.2	4.0	3.6	3.9	5.0	5.4

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

スパイク回収

The spike recovery was evaluated by spiking 3 levels of human HVEM/TNFRSF14 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 82% to 117% with an overall mean recovery of102%.

サンプル値

Sample Matrix	Sample Evaluated	Range (pg/ml)	Detectable (%)	Mean of Detectable (pg/ml)
Serum	30	6823.75-12125.82	100	8948.2

Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of IL-12/IL-23 p40 in this assay. No medical histories were available for the donors.

実験タイプ

Sandwich Elisa

種属反応性

Human

検出方法

Colorimetric

所要時間

120min

標準曲線梯度

7点/3倍

インキュベーション回数

Detectable Sample

可溶性物质的液相样品。例如：血清，血浆，细胞培养上清，组织研磨液等。

サンプル添加量

50 μl/10 μl

製品の特徴

保存条件

2-8 ℃で保存

ターゲット

UNIPROT #

SwissProt: Q92956 Human

別名

HVEML

ATAR

CD270

CD40 like protein precursor

Herpes virus entry mediator A

Herpesvirus entry mediator A

Herpesvirus entry mediator

Herpesvirus entry mediator ligand

HveA

HVEM

展開

機能

Herpesvirus entry mediator (HVEM), also referred to as TR2 (TNF receptor-like molecule) and ATAR (another TRAF-associated receptor), is a type I membrane protein belonging to the TNF/NGF receptor superfamily. In the TNF superfamily nomenclature, HVEM is referred to as TNFRSF14. Human HVEM cDNA encodes a 283 amino acid (aa) protein with a probable 36 aa signal peptide, a 166 aa extracellular domain, a 23 aa transmembrane region and a 58 aa cytoplasmic region. The extracellular domain of HVEM contains several cysteine-rich repeats characteristic of TNF receptor superfamily members. The short cytoplasmic region lacks a death domain present in some TNF receptor family members, but contains a TRAF (TNF receptor-associated factor) interaction domain. HVEM expression has been detected in peripheral blood T cells, B cells, monocytes and in various tissues enriched in lymphoid cells. The extracellular domain of HVEM has been shown to interact directly with the herpes simplex virus envelope glycoprotein D. Two TNF superfamily ligands, including the secreted TNF‑ beta (lymphotoxin alpha ) and the membrane protein LIGHT (lymphotoxins, exhibits inducible expression, and competes with HSV glycoprotein D for HVEM, a receptor expressed by T lymphocytes), have been shown to be the cellular ligands for HVEM. Besides HVEM, LIGHT can also interact with LT beta R, the receptor for lymphotoxin alpha beta heterotrimer.

Human HVEM/TNFRSF14 enzyme-linked immunoassay kit

お問い合わせ

製品概要

製品名

製品タイプ

検出感度

精度

スパイク回収

サンプル値

実験タイプ

種属反応性

検出方法

所要時間

標準曲線梯度

インキュベーション回数

Detectable Sample

サンプル添加量

製品の特徴

保存条件

ターゲット

UNIPROT #

別名

機能

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