Human GDF-15 enzyme-linked immunoassay kit
Catalog# EH0267
Human GDF-15 enzyme-linked immunoassay kit
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ELISA
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Human
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4.10~1000 pg/ml
製品概要
製品名
Human GDF-15 enzyme-linked immunoassay kit
製品タイプ
Elisa Kit
検出感度
0.11 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0080 | 0.0089 | 0.0085 | |
| 4.10 | 0.0208 | 0.0223 | 0.0216 | 0.0131 |
| 10.24 | 0.0417 | 0.0405 | 0.0411 | 0.0327 |
| 25.60 | 0.0956 | 0.0898 | 0.0927 | 0.0843 |
| 64.00 | 0.2179 | 0.2061 | 0.2120 | 0.2036 |
| 160.00 | 0.5291 | 0.4912 | 0.5102 | 0.5017 |
| 400.00 | 1.2370 | 1.1710 | 1.2040 | 1.1956 |
| 1000.00 | 2.5730 | 2.5450 | 2.5590 | 2.5506 |
精度
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 19.6 | 97.1 | 299.2 | 20.6 | 98.6 | 311.8 |
| Standard Deviation | 1.1 | 3.5 | 14.6 | 0.8 | 2.5 | 8.4 |
| Coefficient of Variation (%) | 5.7 | 3.6 | 4.9 | 4.0 | 2.5 | 2.7 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
スパイク回収
The spike recovery was evaluated by spiking 3 levels of human GDF-15 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 105% to 120% with an overall mean recovery of 117%.
サンプル値
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 61.09-290.23 | 100 | 132.88 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated in this assay. No medical histories were available for the donors.
実験タイプ
種属反応性
検出方法
所要時間
標準曲線梯度
インキュベーション回数
2
Detectable Sample
サンプル添加量
製品の特徴
保存条件
2-8 ℃で保存