Human Endostatin/COL18A1 enzyme-linked immunoassay kit(one step)
Catalog# EHY0232
Human Endostatin/COL18A1 enzyme-linked immunoassay kit(one step)
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ELISA
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Human
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1.37~1000 ng/ml
製品概要
製品名
Human Endostatin/COL18A1 enzyme-linked immunoassay kit(one step)
製品タイプ
Elisa Kit
検出感度
0.31 ng/ml (10 μl)
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0066 | 0.0063 | 0.0065 | |
| 1.37 | 0.0117 | 0.0122 | 0.0120 | 0.0055 |
| 4.12 | 0.0255 | 0.0263 | 0.0259 | 0.0195 |
| 12.35 | 0.0680 | 0.0758 | 0.0719 | 0.0655 |
| 37.04 | 0.2051 | 0.2193 | 0.2122 | 0.2058 |
| 111.11 | 0.7228 | 0.6482 | 0.6855 | 0.6791 |
| 333.33 | 1.8520 | 1.8470 | 1.8495 | 1.8431 |
| 1000.00 | 2.8380 | 2.9900 | 2.9140 | 2.9076 |
精度
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (ng/ml) | 18.6 | 90.3 | 323.1 | 25.8 | 108.2 | 374.8 |
| Standard Deviation | 0.8 | 3.7 | 11.3 | 1.1 | 4.7 | 18.7 |
| Coefficient of Variation (%) | 4.4 | 4.1 | 3.5 | 4.2 | 4.3 | 5.0 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
スパイク回収
The spike recovery was evaluated by spiking 3 levels of human Endostatin/COL18A1 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 95% to 120% with an overall mean recovery of 112%.
サンプル値
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 58.87-119.93 | 100 | 76.55 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated in this assay. No medical histories were available for the donors.
実験タイプ
種属反応性
検出方法
所要時間
標準曲線梯度
インキュベーション回数
2
Detectable Sample
サンプル添加量
製品の特徴
保存条件
2-8 ℃で保存