Human Proprotein Convertase 9/PCSK9 enzyme-linked immunoassay kit

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Catalog# EH0071

Human Proprotein Convertase 9/PCSK9 enzyme-linked immunoassay kit

Application

ELISA

Reactivity

Human

Standard curve range

0.78~50 ng/ml

製品概要

製品名

Human Proprotein Convertase 9/PCSK9 enzyme-linked immunoassay kit

製品タイプ

Elisa Kit

検出感度

0.02 ng/ml (50 μl)；0.17 ng/ml (10 μl)

ng/ml	O.D.		Average	Corrected
0.00	0.0510	0.0516	0.0513
0.78	0.1529	0.1621	0.1575	0.1062
1.56	0.2831	0.2918	0.2875	0.2362
3.13	0.5106	0.5036	0.5071	0.4558
6.25	0.8643	0.8867	0.8755	0.8242
12.50	1.6301	1.7365	1.6833	1.6320
25.00	2.7802	2.9585	2.8694	2.8181
50.00	4.4295	4.5187	4.4741	4.4228

精度

	Intra-Assay Precision			Inter-Assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average (ng/ml)	1.0	5.3	15.2	1.1	4.9	17.0
Standard Deviation	0.0	0.3	0.7	0.1	0.4	0.7
Coefficient of Variation (%)	4.8	5.0	4.3	4.2	4.8	4.8

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested Twenty-two times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

スパイク回収

The spike recovery was evaluated by spiking 3 levels of human PCSK9 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 90% to 117% with an overall mean recovery of 104%.

サンプル値

Sample Matrix	Sample Evaluated	Range (ng/ml)	Detectable (%)	Mean of Detectable (ng/ml)
Serum	30	609.35-1634.60	100	1146.64

Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of PCSK9 in this assay. No medical histories were available for the donors.

実験タイプ

Sandwich Elisa

種属反応性

Human

検出方法

Colorimetric

所要時間

120min

標準曲線梯度

7点

インキュベーション回数

Detectable Sample

可溶性物质的液相样品。例如：血清，血浆，细胞培养上清，组织研磨液等。

サンプル添加量

50 μl/10 μl

製品の特徴

保存条件

2-8 ℃で保存

ターゲット

UNIPROT #

SwissProt: Q8NBP7 Human

別名

Convertase subtilisin/kexin type 9 preproprotein

FH3

HCHOLA3

Hypercholesterolemia autosomal dominant 3

LDLCQ1

NARC 1

NARC-1

NARC1

Neural apoptosis regulated convertase 1

Neural apoptosis-regulated convertase 1

展開

機能

Proprotein convertase subtilisin kexin 9 (PCSK9), also named neural apoptosis-regulated convertase 1 (NARC-1), is a member of the proteinase K subfamily of subtilisin-related serine endoproteases. The full-length protein has 692 amino acids, including a signal peptide, a pro- domain, and a catalytic domain. PCSK9 is highly expressed in the liver, intestine, and kidney. It is initially synthesized as a soluble 74 kDa precursor protein. In the endoplasmic reticulum, it undergoes autocatalytic intramolecular cleavage to generate a 14 kDa pro- domain and a 60 kDa catalytic domain. These two domains remain associated when PCSK9 is secreted outside the cells. The primary physiologic function of PCSK9 is to mediate the degradation of low density lipoprotein receptor (LDL R). Early observations indicated that gain-of-function missense mutations in the PCSK9 gene can cause an autosomal dominant form of hypercholesterolemia. The expression of PCSK9 was observed to be up-regulated by the sterol regulatory element binding proteins (SREBPs), a family of transcription factors that are responsible for the upregulation of genes involved in cholesterol and fatty acid metabolism, such as the LDL R gene. Further experimental evidence revealed that in mice, when the PCSK9 gene was knocked out, the number of LDL R in hepatocytes increased, whereas when PCSK9 was over-expressed, the amount of LDL R protein was reduced in the liver. In humans, genetic analyses have shown that individuals who have nonsense or loss-of-function mutations in the PCSK9 gene have significantly lower plasma LDL cholesterol levels. These investigations clearly indicated that PCSK9 plays a key role in reducing the hepatic LDL R levels. Recently, the underlying mechanism has been uncovered: under normal physiologic conditions, the LDL R is internalized on the cell surface and directed to the endosomes in order to be recycled back to the cell surface. PCSK9 binds to the EGF domain of the LDL R and prevents LDL R from being sorted to the endosomes. Instead, the PCSK9/LDL R complex is redistributed to the lysosomes for degradation. As such, PCSK9 regulates the amount of LDL R in the circulation and modulates cholesterol levels. Serum PCSK9 concentrations have been found to be directly associated with cholesterol levels. Since individuals with loss-of-function PCSK9 mutations have strikingly reduced risk of coronary heart diseases, PCSK9 has become an attractive drug target in recent years. One approach is to generate small molecules that are able to interfere with PCSK9 autoactivation and its interaction with LDL R. Other approaches aiming to reduce the amounts of PCSK9 in the circulation, such as small interfering RNAs (siRNAs), have also shown promise.

Human Proprotein Convertase 9/PCSK9 enzyme-linked immunoassay kit

お問い合わせ

製品概要

製品名

製品タイプ

検出感度

精度

スパイク回収

サンプル値

実験タイプ

種属反応性

検出方法

所要時間

標準曲線梯度

インキュベーション回数

Detectable Sample

サンプル添加量

製品の特徴

保存条件

ターゲット

UNIPROT #

別名

機能

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