Human Complement factor D enzyme-linked immunoassay kit(one step)
Catalog# EHY0242
Human Complement factor D enzyme-linked immunoassay kit(one step)
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ELISA
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Human
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0.41~300 ng/ml
製品概要
製品名
Human Complement factor D enzyme-linked immunoassay kit(one step)
製品タイプ
Elisa Kit
検出感度
0.01 ng/ml (10 μl)
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0089 | 0.0083 | 0.0086 | |
| 0.41 | 0.0171 | 0.0172 | 0.0172 | 0.0086 |
| 1.23 | 0.0395 | 0.0399 | 0.0397 | 0.0311 |
| 3.70 | 0.1106 | 0.1147 | 0.1127 | 0.1041 |
| 11.11 | 0.3221 | 0.3323 | 0.3272 | 0.3186 |
| 33.33 | 0.9434 | 0.9330 | 0.9382 | 0.9296 |
| 100.00 | 2.4900 | 2.4320 | 2.4610 | 2.4524 |
| 300.00 | 4.3547 | 4.3549 | 4.3548 | 4.3462 |
精度
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (ng/ml) | 4.9 | 24.7 | 90.1 | 5.1 | 25.9 | 94.7 |
| Standard Deviation | 0.1 | 0.7 | 3.8 | 0.2 | 1.1 | 4.2 |
| Coefficient of Variation (%) | 2.1 | 2.7 | 4.2 | 4.1 | 4.1 | 4.4 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
スパイク回収
The spike recovery was evaluated by spiking 3 levels of human Complement factor D into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 92% to 105% with an overall mean recovery of 98%.
サンプル値
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 433.18-943.01 | 100 | 713.99 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated in this assay. No medical histories were available for the donors.
実験タイプ
種属反応性
検出方法
所要時間
標準曲線梯度
インキュベーション回数
2
Detectable Sample
サンプル添加量
製品の特徴
保存条件
2-8 ℃で保存